[Skip to Content](https://www.sigmaaldrich.com#main-content) [![MilliporeSigma](https://www.sigmaaldrich.com/static/logos/purple/millipore_sigma.svg)](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products ProductsApplicationsServicesResourcesSupport [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Pathogen & Spoilage Testing](https://www.sigmaaldrich.com/US/en/applications/microbiological-testing/pathogen-and-spoilage-testing)Lateral Flow Tests for Immunological Detection in Food and Environmental Samples # Lateral Flow Tests for Immunological Detection in Food and Environmental Samples Lateral flow tests are immunoassays for detecting food pathogens with the ultimate convenience. Covering the major pathogens, the tests act as mini-laboratories and include a built-in control reaction for optimized pathogen testing. Definitive results are delivered in as little as 20 minutes. ## Principle of the lateral flow test for food pathogen detection Immunoassays are designed for the detection of pathogens (including *Salmonella*, *Listeria*, *Campylobacter*, etc.) in food samples and on environmental surfaces. Each device is a self-contained test that requires only the addition of sample, making it an extremely simple and user-friendly method for low to medium volume labs. Once added to the test device, samples are combined with gold-colloid particles coated with capture antibodies specific to the target antigen and drawn across a nitrocellulose membrane via capillary action. If the target antigen is present in the sample, it is captured and bound to the colored particles. The particles continue across the membrane encountering a test line containing antibodies specific to the target antigen followed by a control line containing antibodies specific to the capture antigen coated to the colored particles. If present, the target antigen and its associated particles will become bound to the test line antibodies to form a visually apparent line. The remaining particles will continue to the control line antibodies to form a visually apparent line verifying that the test device worked correctly. ## Benefits of lateral flow tests for detection of food pathogens - Reliable: same accuracy standards as classical detection methods - Comprehensive: tests cover the most relevant food pathogens – *Salmonella, Listeria* spp. *and Listeria monocytogenes, E. coli* 0157, *Campylobacter* - Fast: definitive results within 20 - 30 minutes - Easy-to-use: clear yes/no results after simple sample application - Safe: additional positive control and specially adapted enrichment media for precise, reliable results - Economic: Rapid results save operating costs by speeding up product release while ensuring product reliability ## Lateral flow tests for food and environmental pathogen detection #### VIP® Gold single-step visual lateral flow immunoassay test VIP® Gold (Visual Immuno Precipitate) are designed to be used with proprietary enrichment medium mEHEC. Key tests/pathogens - *Salmonella* - *Listeria* - EHEC (enterohaemorrhagic *E. coli*) ![](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/microbiological-testing/pathogen-and-spoilage-testing/vip-gold.jpg "vip-gold") ### Singlepath® single-step visual lateral flow immunoassay test Singlepath® Lateral Flow Tests is used in combination with our ISO regulated GranuCult® media to ensure optimal test performance. Key tests/pathogens - *Salmonella* - *E. coli O157* - *Listeria monocytogenes* - Campylobacter ![](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/microbiological-testing/pathogen-and-spoilage-testing/lateral-flow-tests.jpg "lateral-flow-tests") ### Food and environmental pathogen validation VIP® GoldSinglepath® *Salmonella*AOAC OMA 999.09 AOAC PTM 031801AOAC PTM 060401 *Listeria*AOAC OMA 997.03 AOAC PTM 060801 EHECAOAC OMA 996.09 Health Canada MFLP-87 *E. coli O157* AOAC PTM 010407 *Campylobacter* AOAC PTM 0120401 ## Materials Sorry, an unexpected error has occurred Response not successful: Received status code 500 __Related Articles__ - [*Lactobacilli* Basics, Testing & Identification](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/clinical-testing-and-diagnostics-manufacturing/bacteriology/lactobacilli) - [Microbial Detection Using Chromogenic and Fluorogenic Culture Media](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/clinical-testing-and-diagnostics-manufacturing/bacteriology/overview-chromogenic-media) - [Identification of Microorganisms Based on Color](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/clinical-testing-and-diagnostics-manufacturing/cytology-and-microscopy/color-identification-microorganisms) - [Culture Method for Beer Quality Control](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/culture-method-beer-quality) - [Detection and Differentiation of Staphylococcus Aureus on Baird Parker Agar](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/detection-and-differentiation) - [Differentiation of Escherichia coli from coliforms](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/differentiation-of-e-coli-coliforms) - [DiluCult™ Gravimetric Dilutor & Sample Homogenizer](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/dilucult-gravimetric-dilutor-homogenizer) - [EN ISO 11133 Microbiological Culture Media](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/en-iso-11133-water-for-the-preparation-and-performance-testing) - [View More](https://www.sigmaaldrich.com/US/en/search/facet-search?focus=sitecontent&term=facet-search) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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