Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

The International Organization for Standardization (ISO), published revised EN ISO 11290-1 and EN ISO 11290-2 standards in 2017. In Part 1, a horizontal method for the detection of Listeria monocytogenes and Listeria spp. is specified, and in Part 2, the enumeration of these organisms in food, animal feed and environmental samples is described. The revised Part 1 edition cancels and replaces the first edition EN ISO 11290-1:1996 and incorporates the amendment 1 from 2004. Part 2 cancels and replaces the first edition of EN ISO 11290-2:1998 and incorporates the amendment 1 from 2004. Compared to the previous edition, the main changes are considered as major. In the revised EN ISO 11290:2017, the scope in both Parts 1 and 2 has been extended to enable also the detection and enumeration of Listeria spp.

Procedure steps for Listeria monocytogenes and Listeria spp. detection according to the EN ISO 11290-1:2017 standard

Procedure steps for Listeria monocytogenes and Listeria spp. detection according to the EN ISO 11290-1:2017 standard

EN ISO 11290-1:2017 at a glance

  • Scope extended to include Listeria spp. detection. The title has changed accordingly.
  • Primary enrichment in half-Fraser broth: incubation changed to 25 h ± 1 h.
  • Secondary enrichment in Fraser broth updated to 24 h ± 2 h.
  • Enriched half-Fraser and Fraser broths may be refrigerated before transfer or isolation on selective agar for max. 72 h.
  • Incubated isolation plates can be refrigerated for a maximum of two days before reading.
  • Microscopic confirmation aspect is optional in the case of use of agar specific for pathogenic Listeria spp.
  • CAMP and catalase tests are optional.
  • New performance characteristics included.

Procedure steps for Listeria monocytogenes and Listeria spp. enumeration according to the revised EN ISO 11290-2:2017 standard

Procedure steps for Listeria monocytogenes and Listeria spp. enumeration according to the revised EN ISO 11290-2:2017 standard

EN ISO 11290-2:2017 at a glance

  • Primary suspension with buffered peptone water, half-Fraser with or without supplements, and all appropriate dilutions are referred to in ISO 6887.
  • Resuscitation step (incubation of initial suspension of sample 1 h ± 5 min at 20 °C ± 2 °C) deleted.
  • Microscopic aspect, CAMP and catalase tests are optional.
  • Enumeration of Listeria spp. is included in the scope. Title is changed accordingly.

As a worldwide leading provider of a broad range of dehydrated granulated and ready-to-use culture media for food, beverages and water microbiology, we closely track and support the development of relevant standards to ensure consumer confidence and safety.

Our GranuCult® granulated media, Readybag® pre-weighed granulated media in ready-to-use bags, ReadyPlate™, and ReadyTube® ready-to-use media are all compliant with EN ISO 11133:2014. All information regarding compliance with additional reference standards are displayed on the product label and in the technical information available on our website.

Compliance to revised ISO 11290:2017

We have implemented all the requirements described in both part 1 and part 2 of the revised EN ISO 11290:2017. For more information, please visit GranuCult™ Granulated Culture Media.

The following culture media and accessories described in the revised EN ISO 11290:2017 are available:

Ordering Information

Product Pack Size Product No.
GranuCult®  Half FRASER (Demi FRASER) Broth (Base) with Antibiotics  acc. ISO 11290 500 g 1.000.250.500
FRASER Listeria Selective Supplement 10 x 1 vial 1.000.930.010
Readybag® Half FRASER (Demi FRASER) Broth with Supplements acc. ISO 11290, 12.5 g, irradiated 60 bags each 12.5 g 1.024.490.060
Readybag® Half FRASER (Demi FRASER) Broth with Supplements acc. ISO 11290, 62 g, irradiated 35 bags each 62 g   1.018.650.001
ReadyTube® 225 Half Fraser ISO 11290 GranuCult® 6 x 225 mL 1.464.760.006
FRASER Broth (Base) acc. ISO 11290 500 g 1.103.980.500
ReadyTube® 10 Fraser Broth ISO 11290 20 x 10 mL 1.462.080.020
Chromocult® Listeria Agar (Base) acc. OTTAVIANI and AGOSTI acc. ISO 11290 500 g 1.004.270.500
Chromocult® Listeria Agar Enrichment Supplement 10 x 1 vial 1.004.390.010
Chromocult® Listeria Agar Selective Supplement Chromocult® Listeria Agar Selective Supplement 10 x 1 vial 1.004.320.010
ReadyPlate™ CHROM Listeria Agar ISO 11290 20 plates 1.461.860.020
PALCAM Listeria-Selective Agar (Base) acc. to VAN NETTEN et al. 500 g 1.117.550.500
PALCAM Listeria Selective-Supplement acc. to van Netten et al. 10 x 1 vial 1.121.220.010
Oxford Listeria-Selective Agar (Base)
500 g 1.070.040.500
Oxford Listeria-Selective Supplement 10 x 1 vial 1.070.060.010
GranuCult® Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP* 500 g 1.072.280.500
GranuCult® Nutrient agar acc. ISO 6579, ISO 10273 and ISO 21528 500 g 1.054.500.500
GranuCult® Tryptic Soy Agar EP, USP, JP, ISO, FDA-BAM** 500 g 1.054.580.500
Maximum Recovery Diluent (Peptone salt solution) 500 g 1.125.350.500
Blood agar base No.2 500 g 1.103.280.500
ReadyTube® 1000 BPW ISO 6579, 6887, 21528, 11290 6 x 1000 mL 1.464.030.006

Other pack sizes available
* 5 kg, 25 kg
** 5 kg