Tables for Vivaspin® Sample Concentrators

Appendix 12, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016

Table A12.1. Maximum sample volumes for different Vivaspin® concentrators

Vivaspin® Fixed angle Swing bucket
500
2
6
20
500 µl
2 ml
6 ml
14 ml
Do not use
3 ml
6 ml
20 ml

 
 
Table A12.2. Recommended maximum centrifugation speed (× g) for different Vivaspin® concentrators

  Vivaspin® 500 Vivaspin® 2 Vivaspin® 6 Vivaspin® 20
Fixed angle        
3000-50 000 MWCO 100 000 MWCO 15 000
15 000
12 000
9000
10 000
6000
8000
6000
Swing bucket        
3000-50 000 MWCO 100 000 MWCO N.A.
N.A.
4000
4000
4000
4000
5000
3000

 
 
Table A12.3. Performance characteristics of Vivaspin® 500

Protein/filter Up to 30× sample concentration1 Recovery
Aprotinin 0.25 mg/ml (6500 MW)    
3000 MWCO 30 min 96%
BSA 1.0 mg/ml (66 000 MW)    
5000 MWCO
10 000 MWCO
30 000 MWCO
15 min
5 min
5 min
96%
96%
96%
IgG 0.25 mg/ml (160 000 MW)    
30 000 MWCO
50 000 MWCO
100 000 MWCO
10 min
10 min
10 min
96%
96%
96%

1 Centrifugation time to achieve an up to 30× sample concentration with a start volume of 500 µl at 20°C.
 
 
Table A12.4. Performance characteristics of Vivaspin® 2

Protein/filter Up to 30× sample concentration1 Recovery
Aprotinin 0.25 mg/ml (6500 MW)    
3000 MWCO 50 min 96%
BSA 1.0 mg/ml (66 000 MW)    
5000 MWCO
10 000 MWCO
30 000 MWCO
12 min
8 min
8 min
98%
98%
97%
IgG 0.25 mg/ml (160 000 MW)    
30 000 MWCO
50 000 MWCO
100 000 MWCO
10 min
10 min
8 min
96%
96%
95%

1 Centrifugation time to achieve an up to 30× sample concentration with a start volume of 2 ml at 20°C.

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