Hybridoma Fusion Partners Cell Lines

Hybridoma Cell Technology

Hybridoma technology involves the production of monoclonal antibodies specific to an antigen of interest. The somatic fusion of B lymphocytes of the spleen with immortal myeloma cells gives rise to a hybridoma cell line that can be perpetually propagated to produce clonally identical antibodies, as these hybridoma cells inherit the indefinite growth properties of myeloma cells and the antibody secretion capabilities of B-lymphocytes. Monoclonal antibodies produced by a unique hybridoma cell line are homogeneous and recognize a single epitope of an antigen.

Monoclonal antibodies produced from hybridoma technology are used in the following applications:

  • Biochemical analysis: Monoclonal antibodies revolutionized laboratory diagnosis. Antibodies are routinely used in  biochemical analysis (RIA, ELISA), immunohistopathology,  and diagnostic imaging (immunoscintigraphy).
  • Immunotherapy: Human, humanized, or chimeric monoclonal antibodies are used in the treatment of cancer, autoimmune diseases, infectious diseases, cardiovascular and other non-oncological treatments, as an adjunct to organ transplantation, and for targeted drug delivery.
  • Protein purification: Monoclonal antibodies are used to purify proteins and are especially useful for the purification of recombinant proteins (immunoaffinity chromatography).
  • Browse our collection of over 400 monoclonal antibody secreting hybridoma cell lines.

Selection of the optimal immortal cell line is critical for achieving the desired attributes of the hybridoma. Lack of suitable fusion partners hampers the applicability of monoclonal antibodies in research and immunotherapy. For example, the application of xenogeneic monoclonal antibodies in therapy and in vivo diagnostics often results in an undesirable human anti-mouse antibody (HAMA) response.

Our selection of fusion partner cell lines include serum- and animal component-free propagation options.  Choose from dozens of options to find the optimal fusion partner for your application and increase the efficiency of hybridoma growth and antibody synthesis.

 

Catalogue No. Cell Line Name Species
85062504 111A3 Mouse
90110506 45.6.TG1.7 Mouse
85062501 53B3 Mouse
93071512 54B3 Mouse
85062503 94A1 Mouse/bovine hybrid
94120825 AFRC 17D1 Horse/mouse hybrid
85082302 BW5147.G.1.4.OUA/R.1 Mouse
90012609 HF2x653 Human/mouse hybrid
89101606 K6H6/B5 Human/mouse hybrid
88112301 N18 Rat/mouse hybrid
88112302 NG108-15 Rat/mouse hybrid
87032003 NG115-401L Rat/mouse hybrid
85110503 NS0 Mouse
03061601 NS0-Serum-Free Mouse
85011427 P3/NS1/1-Ag4.1 Mouse
85011417 P3U1 Mouse
85011401 P3X63Ag8 Mouse
85011420 P3X63Ag8.653 Mouse
03092502 P3X63Ag8.653-TurboDoma Mouse
94120802 P3X63Ag8U.1 Mouse
85072401 SP2/0-Ag14 Mouse
08060101 Sp2/0-Ag14 (AC-free) Mouse
03092501 Sp2/0-Ag14-TurboDoma Mouse
87020507 TK6TGR Human
85051003 U266B1 Human
87020406 V79-HG04 Hamster