[Skip to Content](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products ProductsApplicationsServicesResourcesSupport [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Mammalian Cell Culture](https://www.sigmaaldrich.com/US/en/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/mammalian-cell-culture)Cell Types & Culture Characteristics # Cell Types & Culture Characteristics *ECACC Laboratory Handbook 4th Edition* ## Immortalized Cell Lines Continuous [immortalized cell lines](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-lines-and-specialty-cell-culture/mammalian-cell-lines) are comprised of a single cell type that can be serially propagated in culture either for a limited number of cell divisions (approximately thirty) or otherwise indefinitely. Cell lines of a finite life are usually diploid and maintain some degree of differentiation. The fact that such cell lines senesce after approximately thirty cycles of division means it is essential to establish a system of Master and Working banks in order to maintain such lines for long periods. Continuous immortalized cell lines that can be propagated indefinitely generally have this ability because they have been transformed into tumor cells. Tumor cell lines are often derived from actual clinical tumors, but transformation may also be induced using viral oncogenes or by chemical treatments. Transformed cell lines present the advantage of almost limitless availability, but the disadvantage of having retained very little of the original *in vivo* characteristics. ## Primary Cell Lines [Primary cell lines](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-lines-and-specialty-cell-culture/primary-cells) are derived directly from excised tissue and cultures either as an explant culture or following dissociation into a single cell suspension by enzyme digestion. Such cultures are initially heterogeneous but later become dominated by fibroblasts. The preparation of primary cultures is labor intensive and they can be maintained *in vitro* only for a limited period of time. During their relatively limited lifespan primary cells usually retain many of the differentiated characteristics of the cell *in vivo*. Important Note: Primary cultures by definition have not been passaged, as soon as they are passaged they become a cell line and are no longer primary. ## Stem Cell Lines More recently, [stem cells lines](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-lines-and-specialty-cell-culture/stem-cells) are been a popular cell model used in research due to their unlimited growth characteristics and plasticity. These cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals within the body. These properties provide stem cells with unique capabilities for tissue repair, replacement, and regeneration. ## Cell Morphology Types In terms of growth mode cell cultures take one of two forms, growing either in suspension (as single cells or small free-floating clumps) or as a monolayer that is attached to the tissue culture flask. The form taken by a cell line reflects the tissue from which it was derived. For example, cell lines derived from blood (leukemia, lymphoma) tend to grow in suspension whereas cells derived from solid tissue (lungs, kidney) tend to grow as monolayers. Attached cell lines can be classified as 1) endothelial such as BAE-1, 2) epithelial such as HeLa, 3) neuronal such as SH-SY5Y, or 4) fibroblast such as MRC-5.  __Figure 1. Examples of attached cell types.__ Cells are classified in 4 different cell type categories based on overall morphology 1) Epithelial 2) Endothelial 3) Neuronal or 4) Fibroblast. The European Collection of Authenticated Cell Cultures (ECACC) is one of the world’s largest biological cell banks supplying a diverse range of authenticated cell lines. All 3000+ cell lines can be ordered through us. [Browse the ECACC catalog](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-lines-and-specialty-cell-culture/mammalian-cell-lines). | | | | |-------------------------------------------------------------------------|----------------------------------|----------------| | __Attached Cell Lines__ | | | | __Name__ | __Species and Tissue of Origin__ | __Morphology__ | | MRC-5 | Human Lung | Fibroblast | | [HeLa](https://www.sigmaaldrich.com/US/en/product/sigma/cb_93021013) | Human Cervix | Epithelial | | [Vero](https://www.sigmaaldrich.com/US/en/product/sigma/cb_84113001) | African Green Monkey Kidney | Epithelial | | [NIH3T3](https://www.sigmaaldrich.com/US/en/product/sigma/cb_93061524) | Mouse Embryo | Fibroblast | | [L929](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85011425) | Mouse Connective Tissue | Fibroblast | | C[HO](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85050302) | Chinese Hamster Ovary | Fibroblast | | [BHK-21](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85011433) | Syrian Hamster Kidney | Fibroblast | | [HEK-293](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85120602) | Human Kidney | Epithelial | | [HepG2](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85011430) | Human Liver | Epithelial | | [BAE-1](https://www.sigmaaldrich.com/US/en/product/sigma/cb_88031149) | Bovine Aorta | Endothelial | | [SH-SY5Y](https://www.sigmaaldrich.com/US/en/product/sigma/cb_94030304) | Human Neuroblastoma | Neuronal | | __Suspension Cell Lines__ | | | | __Name__ | __Species and Tissue of Origin__ | __Morphology__ | | [NS0](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85110503) | Mouse Myeloma | Lymphoblast | | [U937](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85011440) | Human Hystiocytic Lymphoma | Lymphoblast | | [HL60](https://www.sigmaaldrich.com/US/en/product/sigma/cb_98070106) | Human Leukemia | Lymphoblast | | [WEHI231](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85022107) | Mouse B-cell Lymphoma | Lymphoblast | | [YAC1](https://www.sigmaaldrich.com/US/en/product/sigma/cb_86022801) | Mouse Lymphoma | Lymphoblast | | [U266B1](https://www.sigmaaldrich.com/US/en/product/sigma/cb_85051003) | Human Myeloma | Lymphoblast | | [Jurkat](https://www.sigmaaldrich.com/US/en/product/sigma/cb_88042803) | Human T-cell Leukemia | Lymphoblast | | [THP-1](https://www.sigmaaldrich.com/US/en/product/sigma/cb_88081201) | Human Monocyte Leukemia | Lymphoblast | Table 1Commonly used cell lines of each cell morphology type ## Phases of Cell Growth It is important to know and record the growth characteristics of the cell line in use before starting any experiments including [cell viability and proliferation](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/cell-viability-and-proliferation) rates. An alteration in cellular growth can indicate a significant problem within the cell line and if undetected, can have detrimental effects on experimental results. A typical growth curve for cultured cells displays a sigmoid pattern of proliferation. The growth phases associated with normal cells are defined as: 1. __Lag Phase__ – at this stage the cells do not divide. During this period the cells adapt to the culture conditions and the length of this phase will depend upon the growth phase of the cell line at the time of subculture and the seeding density. 2. __Logarithmic (Log) Growth Phase__ – cells actively proliferate and an exponential increase in cell density arises. The cell population is considered to be the most viable at this phase; therefore, it is recommended to assess cellular function at this stage. Each cell line will show different cell proliferation kinetics during the log phase and it is therefore the optimal phase for determining the population doubling time. Cells are also generally passaged at late log phase. Passaging cells too late can lead to overcrowding, apoptosis and senescence. 3. __Plateau (or Stationary) Phase__ – cellular proliferation slows down due to the cell population becoming confluent. It is at this stage the number of cells in the active cell cycle drops to 0-10% and the cells are most susceptible to injury. 4. __Decline Phase__ – cell death predominates in this phase and there is a reduction in the number of viable cells. Cell death is not due to the reduction in nutrients, but to the natural progression of the cellular cycle.  __*In Vitro* Age of a Cell Culture__ Two terms are predominantly used to define the age of a cell culture: (i) passage number - indicates the number of times the cell line has been subcultured and (ii) the population doubling (pd) number - indicates the number of cell generations the cell line has undergone i.e. the number of times the cell population has doubled. The *in vitro* age of a cell culture is particularly useful to know for cell lines with a finite lifespan or unstable characteristics that change over time in continuous culture. __Related Articles__ - [Extracellular Matrix Proteins and Tools for Cell Culture Optimization](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/3d-cell-culture/attachment-factors-for-cell-culture) - [Human Serum Albumin (HSA)](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/human-serum-albumin) - [β-Alanine in Cell Culture](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/beta-alanine-cell-culture) - [L-Asparagine in Cell Culture](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/l-asparagine-in-cell-culture) - [L-Ornithine in Cell Culture](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/l-ornithine-cell-culture) - [L-Tyrosine in Cell Culture](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/l-tyrosine-in-cell-culture) - [Corning® High Content Screening Microplates – Frequently Asked Questions](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/corning-high-screening-microplates) - [Using Immortalized 16HBE14o- Human Bronchial Epithelial Cell Lines to Model Respiratory Lung Diseases](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/16hbe-lung-epithelial-cell-lines) - [View More](https://www.sigmaaldrich.com/US/en/search/facet-search?focus=sitecontent&term=facet-search) __Related Product Categories__ - [Mammalian Cell Lines](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-lines-and-specialty-cell-culture/mammalian-cell-lines) - [Primary Cell Culture](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-lines-and-specialty-cell-culture/primary-cells) - [Stem Cells](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-lines-and-specialty-cell-culture/stem-cells) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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