[Skip to Content](https://www.sigmaaldrich.com#main-content) [![MilliporeSigma](https://www.sigmaaldrich.com/static/logos/purple/millipore_sigma.svg)](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Plant Tissue Culture](https://www.sigmaaldrich.com/US/en/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/plant-tissue-culture)Growth Regulators – Plant Tissue Culture Protocol # Growth Regulators – Plant Tissue Culture Protocol We offer a broad range of plant growth regulators specifically tested for plant cell culture. Each product is assayed for physical and chemical characteristics, then is biologically tested following the criteria established for powdered media. Each auxin is tested for enhancement of callus growth and/or root initiation *in vitro*. Each cytokinin is tested for stimulation of shoot production.  The plant growth regulator solutions are sterile filtered through a double 0.2 µm filter unit into sterile bottles. Each lot of plant growth regulator solution is tested for sterility according to specifications established by U.S. Pharmacopeia Vol. XXI, as well as biological testing in plant cell culture using criteria identical to those established for our plant tissue culture media. *FOR LABORATORY USE, PLANT TISSUE CULTURE MEDIA PREPARATION AND PLANT RESEARCH PURPOSES ONLY. NOT FOR USE AS A PLANT GROWTH REGULATOR ON DEVELOPED PLANTS. NOT FOR DRUG OR HOUSEHOLD USES.* ## [](https://www.sigmaaldrich.com)Preparation and Use of Growth Regulators 1. To prepare a 1 mg/mL stock solution, add 100 mg of the plant growth regulator to a 100 mL volumetric flask or other glass container. 2. Add 2-5 mL of solvent to dissolve the powder. Once completely dissolved, adjust the volume with double-processed water (Product No. W3500). Stirring the solution while adding water may be required to keep the material in solution. Store the stock solution as recommended in the tables below. 3. Add 1.0 mL of the stock solution to 1 liter of medium to obtain a final concentration of 1.0 mg/L of the plant growth regulator in the culture medium __(Tables 1-3).__ __Volume of Stock Solution = (Desired Hormone Conc. X Medium Volume) / Stock Solution Conc.__ __Plant Cell Culture Tested Auxins__ are generally used in plant cell culture at a concentration of 0.01-10.0 mg/L. When added in appropriate concentrations, they may regulate cell elongation, tissue swelling, cell division, formation of adventitious roots, inhibition of adventitious and axillary shoot formation, callus initiation and growth, and induction of embryogenesis. __Plant Cell Culture Tested Cytokinins__ are generally used in plant cell culture at a concentration of 0.1-10.0 mg/L. When added in appropriate concentrations, cytokinins may regulate cell division, stimulate axillary and adventitious shoot proliferation, regulate differentiation, inhibit root formation, activate RNA synthesis and stimulate protein and enzyme activity. ## [](https://www.sigmaaldrich.com)Table for Molar Equivalence and Solution Preparation - [Auxins](https://www.sigmaaldrich.com#Auxins) - [Cytokinins](https://www.sigmaaldrich.com#Cytokinins) - [Miscellaneous Plant Growth Regulators](https://www.sigmaaldrich.com#Miscellaneous) ### [](https://www.sigmaaldrich.com)Auxins   __Molar Equivalence____Solution Preparation__ __Product Name____Product No.____Mol. Wt.____µM for 1 mg/L____Solvent____Diluent____Powder Storage____Liquid Storage____Steriliz-__ __ation\*____Working Conc. (mg/L)__ p-Chlorophenoxyacetic acid (4-CPA)[C0413](https://www.sigmaaldrich.com/US/en/product/sigma/C0413)186.65.36EtOH—RT2-8 °CCA0.1-10.0 2,4-Dichlorophenoxyacetic acid[D7299](https://www.sigmaaldrich.com/US/en/product/sigma/D7299)2214.53——RT2-8 °CCA0.01-6.0 2,4-Dichlorophenoxyacetic acid Sodium salt[D6679](https://www.sigmaaldrich.com/US/en/product/sigma/D6679)2434.12Water—RT2-8 °CCA0.01-6.0 Indole-3-acetic acid Free acid (IAA)[I2886](https://www.sigmaaldrich.com/US/en/product/sigma/I2886)175.25.71EtOH/1N NaOHWater-0 °C-0 °CCA/F0.01-3.0 Indole-3-acetic acid Sodium salt[I5148](https://www.sigmaaldrich.com/US/en/product/sigma/I5148)197.25.07WaterWater2-8 °C-0 °CCA/F0.01-3.0 Indole-3-acetic acid methyl ester[I9770](https://www.sigmaaldrich.com/US/en/product/sigma/I9770)189.25.29——2-8 °C2-8 °C—— Indole-3-acetyl-L-aspartic acid[I9387](https://www.sigmaaldrich.com/US/en/product/sigma/I9387)290.33.450.5N NaOHWater-0 °C-0 °CF0.01-5.0 Indole-3-butyric acid (IBA)[I5386](https://www.sigmaaldrich.com/US/en/product/sigma/I5386)203.24.90EtOH/1N NaOHWater2-8 °C-0 °CCA/F0.1-10.0 Indole-3-butyric acid Potassium salt (K-IBA)[I7512](https://www.sigmaaldrich.com/US/en/product/sigma/I7512)241.34.14Water—2-8 °C-0 °CCA/F0.1-10.0 alpha-Naphthaleneacetic acid Free acid (NAA)[N0640](https://www.sigmaaldrich.com/US/en/product/sigma/N0640)186.25.371N NaOHWaterRT2-8 °CCA0.1-10.0 beta-Naphthoxyacetic acid Free acid (NOA)[N3019](https://www.sigmaaldrich.com/US/en/product/sigma/N3019)202.24.951N NaOHWaterRT2-8 °CCA0.1-10.0 Phenylacetic acid (PAA)[P6061](https://www.sigmaaldrich.com/US/en/product/sigma/P6061)136.27.34EtOH—RT2-8 °CCA/F0.1-50.0 Picloram[P5575](https://www.sigmaaldrich.com/US/en/product/sigma/P5575)241.54.14DMSO—RT2-8 °CCA0.01-10.0 2,4,5-Trichlorophenoxyacetic acid (2,4,5-T)[T5785](https://www.sigmaaldrich.com/US/en/product/sigma/T5785)255.53.91EtOH—RT2-8 °CCA0.01-5.0 2,3,5-Triiodobenzoic acid Free acid (TIBA)[T5910](https://www.sigmaaldrich.com/US/en/product/sigma/T5910)499.82.001N NaOHWater-0 °C-0 °CF0.05-5.0 __\*CA__ = coautoclavable with other media components. __F__ = filter sterlize. __CA/F__ = coautoclavable with other media components, however, some loss of activity may occur. This can be compensated for by increasing component concentration. Component may be filter sterilized. ### [](https://www.sigmaaldrich.com)Cytokinins   __Molar Equivalence____Solution Preparation__ __Product Name____Product No.____Mol. Wt.____µM for 1 mg/L____Solvent____Diluent____Powder Storage____Liquid Storage____Steriliz-__ __ation\*____Working Conc. (mg/L)__ Adenine Free base[A5665](https://www.sigmaaldrich.com/US/en/product/sigma/A5665)135.17.401.0 HClWaterRT2-8 °CCA50-250 Adenine hemisulfate Hemisulfate salt[A2545](https://www.sigmaaldrich.com/US/en/product/sigma/A2545)184.25.43Water—RT2-8 °CCA50-250 6-Benzylaminopurine (BA)[B3408](https://www.sigmaaldrich.com/US/en/product/sigma/B3408)225.34.441N NaOHWaterRT2-8 °CCA/F0.1-5.0 6-Benzylaminopurine Hydrochloride[B5920](https://www.sigmaaldrich.com/US/en/product/sigma/B5920)261.73.82Water—RT2-8 °CCA/F0.1-5.0 6-Benzylaminopurine (BA)[B3274](https://www.sigmaaldrich.com/US/en/product/sigma/B3274)225.34.441N NaOHWaterRT2-8 °CCA/F0.1-5.0 N-Benzyl-9-(2-tetrahydropyranyl)adenine (BPA)[B2275](https://www.sigmaaldrich.com/US/en/product/sigma/B2275)309.43.23EtOH—-0 °C-0 °CCA/F0.1-5.0 N-(2-Chloro-4-pyridyl)-N'-phenylurea (4-CPPU)[C2791](https://www.sigmaaldrich.com/US/en/product/sigma/C2791)247.74.04DMSO—2-8 °C2-8 °CF0.001-1.0 6-(gamma,gamma-Dimethylallylamino)purine (2iP)[D7674](https://www.sigmaaldrich.com/US/en/product/sigma/D7674)203.24.921N NaOHWater-0 °C-0 °CCA/F1.0-30.0 6-(gamma,gamma-Dimethylallylamino)purine (2iP)[D5912](https://www.sigmaaldrich.com/US/en/product/sigma/D5912)203.24.921N NaOHWater-0 °C-0 °CCA/F1.0-30.0 1,3-Diphenylurea (DPU)[D7535](https://www.sigmaaldrich.com/US/en/product/sigma/D7535)212.34.71DMSO—RT2-8 °CF0.1-1.0 Kinetin[K0753](https://www.sigmaaldrich.com/US/en/product/sigma/K0753)215.24.651N NaOHWater-0 °C-0 °CCA/F0.1-5.0 Kinetin[K3378](https://www.sigmaaldrich.com/US/en/product/sigma/K3378)215.24.651N NaOHWater-0 °C-0 °CCA/F0.1-5.0 Kinetin[K3253](https://www.sigmaaldrich.com/US/en/product/sigma/K3253)215.24.651N NaOHWater-0 °C-0 °CCA/F0.1-5.0 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea[P6186](https://www.sigmaaldrich.com/US/en/product/sigma/P6186)220.24.54DMSO—RT2-8 °CCA/F0.001-0.05 trans-Zeatin Free base[Z0876](https://www.sigmaaldrich.com/US/en/product/sigma/Z0876)219.24.561N NaOHWater-0 °C-0 °CCA/F0.01-5.0 Zeatin[Z0164](https://www.sigmaaldrich.com/US/en/product/sigma/Z0164)219.24.561N NaOHWater-0 °C-0 °CCA/F0.01-5.0 trans-Zeatin Hydrochloride[Z2753](https://www.sigmaaldrich.com/US/en/product/aldrich/z2753)255.73.91Water—-0 °C-0 °CCA/F0.01-5.0 trans-Zeatin riboside[Z3541](https://www.sigmaaldrich.com/US/en/product/sigma/Z3541)351.42.851N NaOHWater-0 °C-0 °CF0.01-5.0 __\*CA__ = coautoclavable with other media components. __F__ = filter sterlize. __CA/F__ = coautoclavable with other media components, however, some loss of activity may occur. This can be compensated for by increasing component concentration. Component may be filter sterilized. ### [](https://www.sigmaaldrich.com)Miscellaneous Plant Growth Regulators   __Molar Equivalence____Solution Preparation__ __Product Name____Product No.____Mol. Wt.____µM for 1 mg/L____Solvent____Diluent____Powder Storage____Liquid Storage____Steriliz-__ __ation\*____Working Conc. (mg/L)__ (±)-cis,trans-Abscisic acid (ABA)[A1049](https://www.sigmaaldrich.com/US/en/product/sigma/A1049)264.33.781N NaOHWater-0 °C-0 °CCA/F0.1-10.0 Ancymidol[A9431](https://www.sigmaaldrich.com/US/en/product/sigma/A9431)256.33.90DMSO—2-8 °C-0 °CCA/F1.0-10.0 Chlorocholine chloride (CCC)[C4049](https://www.sigmaaldrich.com/US/en/product/sigma/C4049)158.16.33Water—RT2-8 °CFup to 500 3,6-Dichloro-o-anisic acid (Dicamba)[D5417](https://www.sigmaaldrich.com/US/en/product/sigma/D5417)221.04.52EtOH/Water—2-8 °C2-8 °CF0.01-10.0 Gibberellic acid (GA3)[G7645](https://www.sigmaaldrich.com/US/en/product/sigma/G7645)346.42.89EtOH—RT2-8 °CCA/F0.01-5.0 Gibberellic acid Potassium salt (K-GA3)[G1025](https://www.sigmaaldrich.com/US/en/product/sigma/G1025)384.52.60Water—2-8 °C-0 °CCA/F0.01-5.0 Gibberellin A4 Free acid (GA4)[G7276](https://www.sigmaaldrich.com/US/en/product/sigma/G7276)332.43.01EtOH—-0 °C-0 °CF0.01-5.0 (±)-Jasmonic acid[J2500](https://www.sigmaaldrich.com/US/en/product/sigma/J2500)210.34.76EtOH—2-8 °C-0 °CF0.01-100.0 Phloroglucinol[P1178](https://www.sigmaaldrich.com/US/en/product/sigma/P1178)126.17.93Water—RT2-8 °CCA/Fup to 162 N-(Phosphonomethyl)glycine (Glyphosate)[P9556](https://www.sigmaaldrich.com/US/en/product/sigma/P9556)169.15.911N NaOHWaterRT2-8 °CF— Succinic acid 2,2-dimethylhydrazide[S2022](https://www.sigmaaldrich.com/US/en/product/sigma/S2022)160.26.24Water—2-8 °C2-8 °CCA/F0.1-10.0 __\*CA__ = coautoclavable with other media components. __F__ = filter sterlize. __CA/F__ = coautoclavable with other media components, however, some loss of activity may occur. This can be compensated for by increasing component concentration. Component may be filter sterilized. __Related Articles__ - [Classical Plant Media Formulations](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/classic-plant-media) __Related Product Categories__ - [Plant Culture Reagents Applications](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-culture-supplements-and-reagents/plant-culture-reagents) - [Heterocyclic Building Blocks](https://www.sigmaaldrich.com/US/en/products/chemistry-and-biochemicals/building-blocks/heterocyclic-building-blocks) - [Classical Media & Buffers](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-culture-media-and-buffers/classical-media-and-buffers) Top __Sign In To Continue__ To continue reading please sign in or create an account. Sign In__Don't Have An Account?__Register An unknown error has occured.