Selecting Equipment for Purification of GST-tagged Proteins

Extracted from GST Gene Fusion System, GE Healthcare, 2014

The choice of equipment is dependent on the specific purification. Many purification procedures can be carried out using simple equipment and methods, for example, in a step-gradient elution method using a syringe in combination with a prepacked HiTrap column. For more complex elution methods, such as linear gradients, a dedicated system can be used. A system can also be used when the same column is intended to be used for several runs in series. Table 3.4 shows appropriate equipment for different purification processes. See also ÄKTA Laboratory-scale Chromatography Systems: Instrument Management Handbook, GE Healthcare, 29-0108-31.

Table 3.4. Ways of working with standard ÄKTA systems

Table 3.4. Ways of working with standard ÄKTA systems

Fig 3.22. The standard ÄKTA system configurations.

Fig 3.22. The standard ÄKTA system configurations.

Materials

     
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