[Skip to Content](https://www.sigmaaldrich.com#main-content) [![MilliporeSigma](https://www.sigmaaldrich.com/static/logos/purple/millipore_sigma.svg)](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Plant Tissue Culture](https://www.sigmaaldrich.com/US/en/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/plant-tissue-culture)Iron Chelate Solution – Plant Tissue Culture Protocol # Iron Chelate Solution – Plant Tissue Culture Protocol ## To prepare 1L of a 10 X iron chelate solution: 1. Dissolve 0.278 g of ferrous sulfate heptahydrate (Product No. [F8263](https://www.sigmaaldrich.com/US/en/product/SIGMA/F8263)) in 350 ml of tissue culture grade water (Product No. [W3500](https://www.sigmaaldrich.com/US/en/product/SIGMA/W3500)). Heating may be required. 2. Dissolve 0.3725 g of disodium EDTA (Product No. [E6635](https://www.sigmaaldrich.com/US/en/product/SIGMA/E6635)) in 350 ml using tissue culture grade water. Heating may be required. 3. When both components are dissolved, combine the solutions and bring up to the final volume of 1 L using tissue culture grade water. This will yield a clear yellow solution. (Concentrations of components are based upon those present in MS basal salt formulations and utilized in McCown's Woody Plant Medium). ## ALTERNATE METHOD – To prepare 1L of a 200 X iron chelate stock solution: 1. Dissolve 7.45 g of disodium EDTA (Product No. [E6635](https://www.sigmaaldrich.com/US/en/product/SIGMA/E6635)) in 900 ml of tissue culture grade water. A clear solution is obtained in 20 minutes at room temperature (25-27 °C). 2. Gradually add 5.52 g of ferrous sulfate heptahydrate (Product No. [F8263](https://www.sigmaaldrich.com/US/en/product/SIGMA/F8263)). A clear yellow solution is obtained immediately. 3. Store the iron chelate stock solution in an amber bottle to protect it from exposure to light. 4. Use 5 ml of the 200 X iron chelate stock solution to prepare 1 L of culture medium requiring 27.8 mg/L of ferrous sulfate and 37.25 mg/L of disodium salt of EDTA (e.g., Murashige and Skoog, 1962; Gamborg, *et al*., 1968; Lloyd and McCown, 1981). ## Materials Sorry, an unexpected error has occurred Response not successful: Received status code 500 __Related Articles__ - [Murashige and Skoog Plant Cell Culture Media Formulations](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog) - [Orchid Culture Media Formulations](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/orchid-culture-media-formulations) Top __Sign In To Continue__ To continue reading please sign in or create an account. Sign In__Don't Have An Account?__Register An unknown error has occured. - English - EN - Español - ES [Learn More](https://www.sigmaaldrich.com)