Capto MMC ImpRes

Extracted from Multimodal Chromatography (PDF), GE Healthcare, 2013

Capto MMC ImpRes is based on the same ligand as Capto MMC (see Fig 3.6) and as such shows overall similar properties. However, the ligand density is lower (25 to 39 μmol/mL compared with 70 to 90 μmol/mL for Capto MMC). This lower density is optimized for MAb polishing applications. In addition, Capto MMC ImpRes is based on smaller beads (approximately 40 μm compared with approximately 75 μm for Capto MMC). This smaller bead size and the optimized ligand density gives an improved resolution in polishing applications as compared with Capto MMC. The influences of different salt types and additives are the same as for Capto MMC, as discussed above.

Fast mass transfer

The small particle size of Capto MMC ImpRes generally results in a higher DBC and less sensitivity to changes in residence time than Capto MMC.

Salt tolerance

Capto MMC ImpRes has a higher salt tolerance than traditional cation exchangers, which enables loading at higher levels of salt. This is exemplified in the PreDictor plate experiment presented in Figure 3.13, which shows the capacity measured under different salt and pH conditions. However, compared with Capto MMC (not shown), the low ligand density of Capto MMC ImpRes gives a reduced salt tolerance that simplifies elution with salt, leading to higher yield and smaller pool volumes.

Comparison of the effect of salt and pH on the SBC on Capto MMC ImpRes (left) and Capto SP ImpRes (right).

Fig 3.13. Comparison of the effect of salt and pH on the SBC on Capto MMC ImpRes (left) and Capto SP ImpRes (right).

High resolution and high capacity

The smaller particle size of Capto MMC ImpRes, compared with Capto MMC, provides increased resolution. Capto MMC ImpRes also has a high DBC and efficiently removes impurities. In Table 3.4, this is illustrated for MAb purification, and results are compared with those on Capto MMC. At the same load, Capto MMC ImpRes gives better aggregate removal and higher yield. Furthermore, this performance is also maintained at higher load.

Table 3.4. Comparison of the performance of Capto MMC ImpRes and Capto MMC in a Mab purification process using a NaCl gradient for elution

Prototype Load (mg) Cumulated aggregate at 90% yield (%) Yield at 1% aggregate (%)
Capto MMC 281 1.1 88.2
Capto MMC ImpRes 28 0.3 95.9
Capto MMC ImpRes 481 0.5 94.8

1 70% of DBC of the chromatographic media.

Regeneration

For maintenance of the medium, including CIP and storage, see Appendix 2 (Maintenance of media and storage conditions).

Materials

     
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