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[Home](https://www.sigmaaldrich.com/US/en)[Plant Tissue Culture](https://www.sigmaaldrich.com/US/en/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/plant-tissue-culture)Murashige and Skoog Plant Cell Culture Media Formulations
# Murashige and Skoog Plant Cell Culture Media Formulations
Much of plant tissue culture research today still utilizes media developed many years ago by Murashige and Skoog. Below you will find formulation information for many of these media.
- [Basal Salts Mixtures](https://www.sigmaaldrich.com#Basal%20Salts%20Mixtures)
- [Media including Organics](https://www.sigmaaldrich.com#Media%20including%20Organics)
## [](https://www.sigmaaldrich.com)Basal Salts Mixtures
[M5524 – Murashige and Skoog basal salt mixture (MS)](https://www.sigmaaldrich.com/US/en/product/SIGMA/M5524)
[M0654 – Murashige and Skoog basal salt macronutrient solution](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0654)
[M0529 – Murashige and Skoog basal salt micronutrient solution](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0529)
[M2909 – Murashige and Skoog modified basal salt mixture](https://www.sigmaaldrich.com/US/en/product/SIGMA/M2909)
| | | | | |
|------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|
| Component (mg/L) | [M5524](https://www.sigmaaldrich.com/US/en/product/SIGMA/M5524) | [M0654](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0654) | [M0529](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0529) | [M2909](https://www.sigmaaldrich.com/US/en/product/SIGMA/M2909) |
| Aluminum chloride • 6H2O | | | | |
| Ammonium nitrate | 1650.0 | 1650.0 | | |
| Ammonium phosphate monobasic | | | | |
| Ammonium sulfate | | | | |
| Boric acid | 6.2 | | 6.2 | 6.2 |
| Calcium chloride anhydrous | 332.2 | 332.2 | | 332.2 |
| Calcium nitrate | | | | |
| Calcium phosphate tribasic | | | | |
| Cobalt chloride • 6H2O | 0.025 | | 0.025 | 0.025 |
| Cupric sulfate • 5H2O | 0.025 | | 0.025 | 0.025 |
| Na2-EDTA | 37.26 | | 37.3 | 37.26 |
| ZnNa2-EDTA | | | | |
| Ferric chloride • 6H2O | | | | |
| Ferric citrate | | | | |
| Ferric sulfate | | | | |
| Ferric tartrate • 2H2O | | | | |
| Ferrous sulfate • 7H2O | 27.8 | | 27.8 | 27.8 |
| Magnesium sulfate | 180.7 | 180.7 | | 180.7 |
| Manganese chloride • 4H2O | | | | |
| Manganese sulfate • H2O | 16.9 | | 16.9 | 16.9 |
| Molybdenum trioxide | | | | |
| Molybdic acid (sodium salt) • 2H2O | 0.25 | | 0.25 | 0.25 |
| Nickel chloride • 6H2O | | | | |
| Nickel sulfate • 6H2O | | | | |
| Potassium chloride | | | | |
| Potassium iodide | 0.83 | | 0.83 | 0.83 |
| Potassium nitrate | 1900.0 | 1900.0 | | 1900.0 |
| Potassium phosphate monobasic | 170.0 | 170.0 | | 170.0 |
| Potassium sulfate | | | | |
| Sodium nitrate | | | | |
| Sodium phosphate monobasic | | | | |
| Sodium sulfate | | | | |
| Zinc nitrate • 6H2O | | | | |
| Zinc sulfate • 7H2O | 8.6 | | 8.6 | 8.6 |
Organics (mg/L)[M5524](https://www.sigmaaldrich.com/US/en/product/SIGMA/M5524)[M0654](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0654)[M0529](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0529)[M2909](https://www.sigmaaldrich.com/US/en/product/SIGMA/M2909)
Activated charcoal
Adenine hemisulfate
Agar
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma-
Dimethylallylaminopurine
Glycine (free base)
Indole-3-acetic acid
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid)
Peptone
Pyridoxine • HCl
Sucrose
Thiamine • HCl
| | | | | |
|--------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|
| | [M5524](https://www.sigmaaldrich.com/US/en/product/SIGMA/M5524) | [M0654](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0654) | [M0529](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0529) | [M2909](https://www.sigmaaldrich.com/US/en/product/SIGMA/M2909) |
| Grams of powder to prepare 1 L | 4.3 | n/a | n/a | 2.7 |
| pH ± 0.5 at room temperature | 3.9 | 4.3 | 3.2 | 3.9 |
## [](https://www.sigmaaldrich.com)Media including Organics
[M6899 – Murashige and Skoog basal salts with minimal organics](https://www.sigmaaldrich.com/US/en/product/SIGMA/M6899)
[M5519 – Murashige and Skoog basal medium](https://www.sigmaaldrich.com/US/en/product/SIGMA/M5519)
[M0404 – Murashige and Skoog basal medium with Gamborg's vitamins](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0404)
[M9274 – Murashige and Skoog basal medium with sucrose and agar](https://www.sigmaaldrich.com/US/en/product/SIGMA/M9274)
[M7024 – Murashige and Skoog shoot multiplication medium A](https://www.sigmaaldrich.com/US/en/product/SIGMA/M7024)
| | | | | | |
|------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|
| Component (mg/L) | [M6899](https://www.sigmaaldrich.com/US/en/product/SIGMA/M6899) | [M5519](https://www.sigmaaldrich.com/US/en/product/SIGMA/M5519) | [M0404](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0404) | [M9274](https://www.sigmaaldrich.com/US/en/product/SIGMA/M9274) | [M7024](https://www.sigmaaldrich.com/US/en/product/SIGMA/M7024) |
| Aluminum chloride • 6H2O | | | | | |
| Ammonium nitrate | 1650.0 | 1650.0 | 1650.0 | 1650.0 | 1650.0 |
| Ammonium phosphate monobasic | | | | | |
| Ammonium sulfate | | | | | |
| Boric acid | 6.2 | 6.2 | 6.2 | 6.2 | 6.2 |
| Calcium chloride anhydrous | 332.2 | 332.2 | 332.2 | 332.2 | 332.2 |
| Calcium nitrate | | | | | |
| Calcium phosphate tribasic | | | | | |
| Cobalt chloride • 6H2O | 0.025 | 0.025 | 0.025 | 0.025 | 0.025 |
| Cupric sulfate • 5H2O | 0.025 | 0.025 | 0.025 | 0.025 | 0.025 |
| Na2-EDTA | 37.26 | 37.26 | 37.26 | 37.26 | 37.26 |
| ZnNa2-EDTA | | | | | |
| Ferric chloride • 6H2O | | | | | |
| Ferric citrate | | | | | |
| Ferric sulfate | | | | | |
| Ferric tartrate • 2H2O | | | | | |
| Ferrous sulfate • 7H2O | 27.8 | 27.8 | 27.8 | 27.8 | 27.8 |
| Magnesium sulfate | 180.7 | 180.7 | 180.7 | 180.7 | 180.7 |
| Manganese chloride • 4H2O | | | | | |
| Manganese sulfate • H2O | 16.9 | 16.9 | 16.9 | 16.9 | 16.9 |
| Molybdenum trioxide | | | | | |
| Molybdic acid (sodium salt) • 2H2O | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Nickel chloride • 6H2O | | | | | |
| Nickel sulfate • 6H2O | | | | | |
| Potassium chloride | | | | | |
| Potassium iodide | 0.83 | 0.83 | 0.83 | 0.83 | 0.83 |
| Potassium nitrate | 1900.0 | 1900.0 | 1900.0 | 1900.0 | 1900.0 |
| Potassium phosphate monobasic | 170.0 | 170.0 | 170.0 | 170.0 | 170.0 |
| Potassium sulfate | | | | | |
| Sodium nitrate | | | | | |
| Sodium phosphate monobasic | | | | | 148.0 |
| Sodium sulfate | | | | | |
| Zinc nitrate • 6H2O | | | | | |
| Zinc sulfate • 7H2O | 8.6 | 8.6 | 8.6 | 8.6 | 8.6 |
Organics (mg/L)[M6899](https://www.sigmaaldrich.com/US/en/product/SIGMA/M6899)[M5519](https://www.sigmaaldrich.com/US/en/product/SIGMA/M5519)[M0404](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0404)[M9274](https://www.sigmaaldrich.com/US/en/product/SIGMA/M9274)[M7024](https://www.sigmaaldrich.com/US/en/product/SIGMA/M7024)
Activated charcoal
Adenine hemisulfate 80.0
Agar 8000.0
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma-
Dimethylallylaminopurine 30.0
Glycine (free base) 2.0 2.0
Indole-3-acetic acid 0.3
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol100.0100.0100.0100.0100.0
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid) 0.51.00.5
Peptone
Pyridoxine • HCl 0.51.00.5
Sucrose 30,00030,000
Thiamine • HCl0.40.110.00.10.4
| | | | | | |
|--------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|-----------------------------------------------------------------|
| | [M6899](https://www.sigmaaldrich.com/US/en/product/SIGMA/M6899) | [M5519](https://www.sigmaaldrich.com/US/en/product/SIGMA/M5519) | [M0404](https://www.sigmaaldrich.com/US/en/product/SIGMA/M0404) | [M9274](https://www.sigmaaldrich.com/US/en/product/SIGMA/M9274) | [M7024](https://www.sigmaaldrich.com/US/en/product/SIGMA/M7024) |
| Grams of powder to prepare 1 L | 4.4 | 4.4 | 4.4 | 42.4 | 34.7 |
| pH ± 0.5 at room temperature | 3.9 | 3.9 | 3.8 | 5.0 | 3.9 |
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