[Skip to Content](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Plant Tissue Culture](https://www.sigmaaldrich.com/US/en/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-cell-type/plant-tissue-culture)Silver Thiosulfate – Plant Tissue Culture Protocol # Silver Thiosulfate – Plant Tissue Culture Protocol Prepare a 0.1 M [Sodium Thiosulfate](https://www.sigmaaldrich.com/US/en/product/sigma/S7026) (STS) stock solution by dissolving 1.58 g of [Sodium Thiosulfate](https://www.sigmaaldrich.com/US/en/product/sigma/S7026) (Product No. [S7026](https://www.sigmaaldrich.com/US/en/product/sigma/S7026)) into 100 mL of water. Prepare a 0.1 M [Silver Nitrate](https://www.sigmaaldrich.com/US/en/product/sigma/S7276) stock solution by dissolving 1.7 g of [Silver Nitrate](https://www.sigmaaldrich.com/US/en/product/sigma/S7276) (Product No. [S7276](https://www.sigmaaldrich.com/US/en/product/sigma/S7276)) into 100 mL of water. Store the stock solutions in the dark until needed to prepare the STS. In general, the STS is prepared with a molar ratio between silver and thiosulfate of 1:4, respectively. Nearly all of the silver present in the solution is in the form of \[Ag(S2O3)2]3-, the active complex for ethylene effect inhibition. Prepare a 0.02 M STS by slowly pouring 20 mL of 0.1 M [silver nitrate](https://www.sigmaaldrich.com/US/en/product/sigma/S7276) stock solution into 80 mL of 0.1 M [sodium thiosulfate](https://www.sigmaaldrich.com/US/en/product/sigma/S7026) stock solution. The STS can be stored in the refrigerator for up to a month. However, preparation of the STS just prior to use is recommended. ## Materials Sorry, an unexpected error has occurred Response not successful: Received status code 500 __Related Articles__ - [Antibiotics in Plant Tissue Culture Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/antibiotics) - [Explant Sterilization – Plant Tissue Culture Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/explant-sterilization) - [Gelling Agents – Plant Tissue Culture Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/gelling-agents) - [Media Preparation – Plant Tissue Culture Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/media-preparation) - [Pathology Media – Plant Tissue Culture Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/pathology-media) - [Phycology Media in Plant Tissue Culture](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/phycology-media) - [Vitamin Mixtures – Plant Tissue Culture Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/vitamin-mixtures) - [Genome Editing in Plants with CRISPR/Cas9](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/advanced-gene-editing/genome-editing-in-plants-with-crispr-cas9) - [View More](https://www.sigmaaldrich.com/US/en/search/facet-search?focus=sitecontent&term=facet-search) __Related Product Categories__ - [Salts](https://www.sigmaaldrich.com/US/en/products/chemistry-and-biochemicals/lab-chemicals/salts) Top __Sign In To Continue__ To continue reading please sign in or create an account. Sign In__Don't Have An Account?__Register An unknown error has occured. - English - EN - Español - ES [Learn More](https://www.sigmaaldrich.com)