10X Phosphate-Buffered Saline (PBS) for Western Blotting

This calculator enables the accurate preparation of this 10X phosphate-buffered saline (PBS) for any volume that you need. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed.

Solution concentrations:
[NaCl]: 1.37 M
[KCl]: 27 mM
[Na2HPO4]: 100 mM
[KH2PO4]: 18 mM
 

1 L Recipe Desired Volume: mL    
17.8 g of Na2HPO4 • 2H2O (FW 177.99) (71643)   g of Na2HPO4 • 2H2O
2.4 g of KH2PO4 (FW 136.09) (P9791)   g of KH2PO4
80 g of NaCl (FW 58.44) (71376)   g of NaCl
2 g of KCl (FW 74.55) (P9541)   g of KCl
Adjust final volume to 1 L with Milli-Q® Water Adjust final volume to   mL with Milli-Q® Water
  The pH of the 10X stock will be approximately 6.8, but when diluted to 1x PBS the pH should change to 7.4. When making buffer solutions, it is good practice to measure the pH using a pH meter. If necessary, the pH can be adjusted using hydrochloric acid or sodium hydroxide.

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