Tris-Buffered Saline (TBS) 10X Stock Solution for Western Blots

This calculator enables the preparation of a 10X TBS wash buffer stock solution, whether you are preparing enough for a single experiment or for the entire lab. Input your desired volume and click the CALCULATE button. The right side of the table will populate with the correct amounts of each reagent needed for your desired total final volume.

Solution concentrations:
[Tris]: 200 mM
[NaCl]: 1500 mM
 

1 L Recipe Desired Volume: mL    
24 g of Tris Base (FW 121.14) (93362)   g of Tris Base
88 g of NaCl (FW 58.44) (71376)   g of NaCl
Dissolve in 900 mL Milli-Q® water. Adjust pH to 7.6 with 12 N HCl (H1758).
Adjust pH to 7.6 with 12 N HCl (H1758). Adjust final volume to   mL with Milli-Q® Water
Adjust final volume to 1 L with Milli-Q® Water Adjust pH to 7.6 with 12 N HCl before adjusting to final volume with Milli-Q® water.
  Prepared TBS is stable at 4 °C for 3 months.

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