1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST) for Western Blotting

This calculator enables the accurate preparation of this 1X phosphate-buffered saline with Tween® detergent (PBST) wash buffer for any volume that you need. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed.

Solution concentrations:
[NaCl]: 137 mM
[KCl]: 2.7 mM
[Na2HPO4]: 10 mM
[KH2PO4]: 1.8 mM
[Tween® 20]: 0.1% (w/v)

1 L Recipe Desired Volume: mL    
100 mL of 10X PBS Stock Solution   mL of 10X PBS Stock Solution
900 mL of Milli-Q® Water   mL of Milli-Q® Water
1 mL of Tween® 20 detergent (P9416)   mL of Tween® 20 detergent

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