1X PBS (Phosphate-Buffered Saline) Concentration Recipe Calculator

Preparing PBS 1x by Volume

This calculator enables the accurate preparation of this 1X phosphate-buffered saline (PBS) wash buffer for any milliliter volume that you need. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed to create this common buffer solution for Western blotting.

1x PBS Solution Concentrations:

  • [NaCl]: 137 mM
  • [KCl]: 2.7 mM
  • [Na2HPO4]: 10 mM
  • [KH2PO4]: 1.8 mM

Recipe Calculator

1 L Recipe Desired Volume: mL    
100 mL of 10X PBS Stock Solution   mL of 10X PBS Stock Solution
900 mL of Milli-Q® Water   mL of Milli-Q® Water
  The resultant 1X PBS has a final concentration of 137 mM NaCl, 10 mM phosphate, 2.7 mM KCl; pH 7.4.

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