1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST) for Western Blotting

This calculator enables the accurate preparation of this 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST) wash buffer whether you are making enough for a single experiment or for the entire lab. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed.

Solution concentrations:
[Tris]: 20 mM 
[NaCl]: 150 mM
[Tween® 20]: 0.1% (w/v)
 

1 L Recipe Desired Volume: mL    
100 mL of 10X TBS Stock Solution   mL of 10X TBS Stock Solution
900 mL of Milli-Q® Water   mL of Milli-Q® Water
1 mL of Tween® 20 detergent (P9416)   mL of Tween® 20 detergent

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