1X Tris-Buffered Saline (TBS) Working Solution for Western Blotting

This calculator enables the accurate preparation of this Tris-buffered saline (TBS) working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed.

Solution concentrations:
[Tris]: 20 mM
[NaCl]: 150 mM

1 L Recipe Desired Volume: mL    
100 mL of 10X TBS Stock Solution   mL of 10X TBS Stock Solution
900 mL of Milli-Q® Water   mL of Milli-Q® Water
  The resultant 1X TBS working solution has a final concentration of 150 mM NaCl, 50 mM Tris; pH 7.6

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