4X SDS Sample Loading Buffer for Western Blotting

This calculator enables the accurate preparation of this 4X SDS sample loading buffer for any volume that you need. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed.

Solution concentrations:
[Tris-HCl]: 0.2 M
[DTT]: 0.4 M
[SDS]: 277 mM, 8.0% (w/v)
[Bromophenol blue]: 6 mM
[Glycerol]: 4.3 M

7.5 mL Recipe   Desired Volume: mL    
1.5 mL 1 M Tris-HCl pH 6.5 (20-160)
    mL 1 M Tris-HCl pH 6.5
3 mL of 1 M DTT (dithiothreitol) (43816)
  mL of 1 M DTT (dithiothreitol)
0.6 g of SDS (sodium dodecyl sulfate) (L3771)   g of SDS (sodium dodecyl sulfate)
30 mg of bromophenol blue (B0126)   mg of bromophenol blue
2.4 mL of glycerol (G5516)   mL of glycerol
Adjust final volume to 7.5 mL with Milli-Q® Water Adjust final volume to   mL with Milli-Q® Water
  Make 500 µL aliquots and store at -20 °C.

Note: Should the solution appear orange/yellow, it may be necessary to raise the pH by adding a suitable base, such as 5 M NaOH, dropwise.

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