Acidic Western Blot Stripping Solution

This calculator enables the accurate preparation of this acidic Western blot stripping buffer for any volume that you need. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed.
 

1 L Recipe Desired Volume: mL    
2.8 g of Glycine (G2879)   g of Glycine
10 g of SDS (L3771)   g of SDS
Adjust pH to 2.2 with HCl (H1758) Adjust pH to 2.2 with HCl
Adjust to final volume with Milli-Q® Water Adjust to final volume with Milli-Q® Water
  Procedure
  1. Place the blot in stripping solution and agitate for 30 minutes.
  2. Place the blot in 1X PBS and agitate for 10 minutes. Replace buffer and repeat this wash step.
  3. Proceed to the blocking step for the next immunodetection.

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