Bovine Serum Albumin (BSA) Blocking Buffer Recipe

Bovine serum albumin (BSA) blocking buffer is ideal for saturating excess protein-binding sites on membranes and microplates for Western blotting and ELISA applications, respectively. Typically, 1-3% BSA is sufficient for most applications. To make 100 mL of a 1% BSA blocking buffer, dissolve 1 g of BSA in 100 mL of TBST.  

The BSA blocking buffer recipe calculator enables the accurate preparation of BSA blocking solution whether you are making enough for a single experiment or for the entire lab. Input your desired concentration and volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.
 

BSA Blocking Buffer Recipe Calculator

1% in 100 mL Recipe BSA: % at Desired Volume: mL    
1 g BSA (A5611) g BSA
in 100 mL of 1X TBST in mL of 1X TBST

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