Stock Components for Western Blot Stripping Solutions

This calculator enables the accurate preparation of stock components for the detergent-based Western blot stripping solution for any volume that you need. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed.
 

0.5 M Tris-HCl pH 6.8 — 100 mL Recipe Desired Volume: mL    
6.06 g of Tris Base (FW 121.1) (93362)   g of Tris Base
60 mL of Milli-Q® Water   mL of Milli-Q® Water
Adjust to pH 6.8 with 12 N HCl (H1758) Adjust to pH 6.8 with 12 N HCl
Adjust final volume to 100 mL with Milli-Q® Water Adjust final volume to   mL with Milli-Q® Water
10% SDS — 100 mL Recipe Desired Volume: mL    
10 g of SDS (L3771)   g of SDS
Adjust final volume to 100 mL with Milli-Q® Water Adjust final volume to   mL with Milli-Q® Water
  Dissolve by gently stirring; slowly adjust to final volume with Milli-Q® water.


Other recipes for Western Blotting Buffers & Solutions