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EXtrelut® NT simplifies liquid-liquid extraction by replacing separation funnels. Using a single step is more efficient and provides solvent, material, and time savings in comparison to classical funnel separation. ### Specifications of EXtrelut® NT __Characteristics__ Specially processed, wide-pore diatomaceous earth with a high pore volume Chemically inert Naturally occurring product __Capacity limit with aqueous sample__ [EXtrelut® NT1](https://www.sigmaaldrich.com/US/en/product/mm/115094) 1 mL __without any breakthrough__ [EXtrelut® NT3](https://www.sigmaaldrich.com/US/en/product/mm/115095) 3 mL [EXtrelut® NT20](https://www.sigmaaldrich.com/US/en/product/mm/115096) 20 mL __pH range__ pH 1-10 Uniform batch-to-batch quality ## EXtrelut® NT Pre-packed Column Si è verificato un errore inatteso. Response not successful: Received status code 500 ## EXtrelut® NT Packing Material Si è verificato un errore inatteso. Response not successful: Received status code 500 ## EXtrelut® NT Accessories Si è verificato un errore inatteso. Response not successful: Received status code 500 ## Benefits of Extrelut® NT over LLE - Minimal solvent usage - Simple method - Higher sample capacity and throughput - Emulsion free extracts - Higher purity, suitable for trace analysis EXtrelut® NT SLE sorbent is extremely versatile and can be used for biological samples, water analysis, food and beverage, and environmental applications. Any LLE of aqueous samples can be easily replaced with Extrelut® NT supported liquid extraction. With its easy-to-use working principle a higher recovery and cleaner extraction can be achieved. The aqueous sample is simply applied to the LLE of aqueous samples. It distributes itself in the form of a thin film over the chemically inert matrix and thus acts as a stationary phase. Subsequently, elution takes place using organic solvents that are non miscible with water, solvents like e.g. diethyl ether, ethyl acetate or halogenated hydrocarbons. All the lipophilic substances are extracted from the aqueous into the organic phase. During this process the aqueous phase remains on the stationary phase. The eluate is free from emulsions and can be evaporated for further analysis. __The capacity of EXtrelut® NT pre-packed columns for aqueous samples are specified by the designation.__ Significantly smaller samples must be appropriately diluted. If larger volumes are applied, the columns are overloaded; water breaks through into the solvent. Elution is carried out with 2-3 times the sample volume. The liquid may simply be allowed to run through the column by gravity. The column outlet cannula regulates the solvent flow appropriately. ### Important EXtrelut® NT extraction parameters EXtrelut NT® extraction columnsOutlet cannulaeMaximum sample volume (mL)Waiting period (min)Recommended elution volume (mL) EXtrelut® NT10.60 x 30 mm15 – 106 EXtrelut® NT30.60 x 30 mm35 – 1015 EXtrelut® NT20 0.70 x 30 mm2010 – 1540 1. In order to prevent water breaking through the sample, don’t overload the column. 2. Shorter waiting times can affect the recoveries adversely. 3. The recommended sample volumes must be adhered to. Solutions of smaller volumes must be diluted to give indicated volumes. ### Application: HILIC Separation of Antiepileptic Drugs (AEDs) in Serum after Extrelut® NT SLE EXtrelut® NT has been used for quite some time within research, for the sample preparation of urine, whole blood, plasma, serum, gastric juice, liquor, amniotic fluid, feces, animal and plant tissue. Other applications are in the areas of environmental and residue analysis, e.g. the analysis of industrial, domestic and waste water. The fractionated elution of acidic and basic substances (e.g. drugs and their metabolites) from body fluids is also possible. HPLC: LaChrom® system column: LiChrospher® RP-select B (5 µm) LiChroCART® 250-4 mobile phase: A: Water LiChrosolv® Acetonitrile LiChrosolv® (1+1) B: Water LiChrosolv® gradient: Time \[min] % A % B 0 10 90 30 60 40 44 60 40 44.1 100 0 50 100 0 51 10 90 75 10 90 flow: 1 mL/min     temperature: 30 °C     detection: UV 205 nm      __Figure 1.__ HILIC separation of antiepileptic drugs (AEDs) in serum after Extrelut® NT SLE. ### Determination of Antiepileptic Drugs (AEDs) in Serum 500 µL serum 500 µL phosphate buffer* __Apply in sequence onto the column__ EXtrelut® NT1 __Wait 8 minutes__ 1 mL dichloromethane / 2-propanol (9+1) __Wait 10 minutes then elute with__ 6 mL dichloromethane / 2-propanol (9+1) __Evaporate to dryness under nitrogen stream__ Redissolve residue in 1 mL of methanol   Inject 10 µL into HPLC column \* 17.6 g NaH2PO4, 4.5 g NA2HPO4 2 H2O, 1.5 g NaN3, dissolve in 1 L water (pH 6.0-6.1) | | | | |-------------------------------------------|----------|-------------------| | Recoveries \[mean values N = 3] | | | | Ethosuximide* | 14.1 min | 84 ± 7 % | | Primidone | 19.4 min | 100 ± 2 % | | a-Methyl-apropylsuccinimide | 22.5 min | Internal Standard | | Phenobarbital | 26.9 min | 96 ± 2 % | | Hexobarbital | 34.2 min | 99 ± 2 % | | Carbamazepine | 36.4 min | 97 ± 1 % | | Phenytoin | 38.0 min | 100 ± 1 % | | \*Ethosuximide is volatile on evaporation | | | __Related Articles__ - [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) - [The Effect of Container Materials on Production of Light Induced Off-Flavors in Milk – A Study Using SPME-GC-MS](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/container-materials) - [Analysis of Pesticide Residues in Ginger Powder Using QuEChERS Extraction and Clean-up with a Novel Dual Layer SPE Cartridge](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-ginger-powder) - [Analysis of Pesticide Residues in Pistachios Using QuEChERS Extraction and Cleanup with Supel™ QuE Z-Sep+](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-pistachios) - [HybridSPE-Phospholipid Technology](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/hybridspe-precipitation-technology) - [Impact of Mobile Phase Additives on LC-MS Sensitivity, Demonstrated using Spice Cannabinoids](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lcms-spice-cannabinoids) - [Analysis of Drugs of Abuse in Urine After Cleanup with HLB Solid Phase Extraction 96-well Plates](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-extraction/analysis-of-drugs-of-abuse-in-urine-after-cleanup-with-hlb) - [Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma Samples](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/solid-phase-extraction/chiral-and-achiral-lc-ms-analysis-of-warfarin-in-plasma-samples) - [View More](https://www.sigmaaldrich.com/US/en/search/facet-search?focus=sitecontent&term=facet-search) Top __Autenticati per continuare__ Per continuare a leggere, autenticati o crea un account. 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