[Skip to Content](https://www.sigmaaldrich.com#main-content) [![MilliporeSigma](https://www.sigmaaldrich.com/static/logos/purple/millipore_sigma.svg)](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products ProductsApplicationsServicesResourcesSupport [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)DNA and siRNA Custom iScale Oligos™ # DNA and siRNA Custom iScale Oligos™ __Section Overview__ - [Why are scalable oligos useful?](https://www.sigmaaldrich.com#scalable-oligos) - [Scale Oligos DNA Specifications](https://www.sigmaaldrich.com#scale-oligos-dna-specifications) - [Commercial and cGMP Oligo Capabilities](https://www.sigmaaldrich.com#oligo-capabilities) - [siRNA](https://www.sigmaaldrich.com#sirna) - [iScale Oligos siRNA Specifications](https://www.sigmaaldrich.com#iscale-oligos-sirna-specifications) - [Demonstrated Use *in vivo*](https://www.sigmaaldrich.com#demonstrated-use-in-vivo) - [Related Products](https://www.sigmaaldrich.com#related-products) ![](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/categories/custom-products/iscale-pic-vab.jpg "Custom iScale Oligo vials") ## WHY ARE SCALABLE OLIGOS USEFUL?[](https://www.sigmaaldrich.com) Scalable oligos are crucial because they allow for flexible production in varying quantities to meet specific research or commercial needs. This scalability ensures efficient resource utilization, cost-effectiveness, and adaptability to project demands. Whether it's small-scale experiments or large-scale commercial applications, scalable oligos offer the versatility and convenience required for streamlined workflows and optimized outcomes in research and applied genomics. iScale Oligos DNA and siRNA offer scalable production of milligram to gram quantities of custom DNA and siRNA oligonucleotides. This scalable manufacturing capability caters to specialized needs in commercial applications, ensuring efficient and cost-effective production of high-quality oligos for research, diagnostics, lab developed tests (LDTs) and therapeutic development. ## Scalable Custom DNA Oligos With 35+ years expertise in oligo manufacturing, we are a leading supplier of scalable custom oligonucleotides globally. Milligram and gram quantities of Custom DNA Oligos are for *in vivo*, high-throughput, and commercial projects (life science research tools, molecular diagnostics, and laboratory developed tests). iScale custom oligos are batch validated under stringent QMS control to meet unique customer needs. [Order DNA (mg)](https://www.sigmaaldrich.com/US/en/configurators/tube) * * * ## Related Products Sorry, an unexpected error has occurred Response not successful: Received status code 500 * * * ### Custom DNA oligo benefits - Oligonucleotide quantities available to meet your experimental and commercial needs with fast and efficient global manufacturing scalability - Direct consultation with our scientific team to set specifications for your application - State-of-the-art analytical laboratory, ensuring a tightly-controlled, high-quality product ## Scalable custom oligo DNA specifications[](https://www.sigmaaldrich.com) iScale oligos are available in a wide range of sequence lengths, quantities, purifications and formats. For anything marked as ‘Inquire’ below or if you have custom oligo needs that are different from the general specifications presented, please send a request to [dnaoligos@milliporesigma.com](mailto:dnaoligos@milliporesigma.com). Quantities - 10, 25, 50, 100, 250 & 500 mg - 1, 2, 5, 10, & 15 g (inquire for larger quantities) [Purification](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/dna-and-rna-purification/best-purification) - Desalt, RP-HPLC & *in vivo* Treatment Sequence Lengths - 10 to 55 bases (inquire for longer lengths) [Modifications](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/qpcr/custom-dna-oligos-modifications) - 6-FAM, Biotin, Phosphate, Amino-Modifiers & others - Inquire for feasibility of Locked Nucleic Acid & other modifications [Quality Control](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/custom-dna-oligos-qc-qa) - 100% mass spectrometry - Analytical HPLC available - Certificate of Analysis available Format - Supplied dry or in solution in tubes or plates - Pooling, aliquoting & normalization available (inquire for feasibility) Services - __Salt Exchange__: replaces toxic ammonium ions from the synthesis with physiologically-tolerable sodium ions. Included for all purification types. - __Filtration__: minimizes bacteria, molds and other contaminants. Included for *in vivo* treatment purification. - __Endotoxin testing__: ensures gram-negative LPS is below an acceptable threshold, otherwise the host may die from toxic shock as initiated by and immune cascade. Available for *in vivo* treatment purification. ### Useful Applications for scalable custom oligos - Antisense - Binding studies - High-throughput PCR/qPCR - Sanger Sequencing & NGS (non-adapters) - X-ray crystallographic structure determination - Immunostimulatory assays - Microarray production ### Custom oligo manufacturing Quality Assurance At the foundation of our oligo manufacturing processes is a robust [Quality Management System (QMS)](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/qpcr/oligonucleotide-quality-assurance-quality-management-system-components) that drives compliance to our following [Quality Registrations](https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/iso-certificates): - ISO 9001:2015 for manufacturing research-grade oligonucleotides - ISO 13485:2016 for manufacturing diagnostic-grade oligonucleotides - ISO 14001:2015 for effective environmental management This quality culture is integrated into all aspects of our custom oligo business. [Key components of our QMS](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/qpcr/oligonucleotide-quality-assurance-quality-management-system-components) include: - Certificates of analysis - Document control - Change notification - Vendor management - Business agreements - Corrective and preventive actions Each component drives quality assurance, and for verification, we routinely host rigorous registrar, internal, and customer audits. [Order DNA (mg)](https://www.sigmaaldrich.com/US/en/configurators/tube) * * * ## Commercial and cGMP Oligo Capabilities[](https://www.sigmaaldrich.com) As part of our [Commercial Oligos](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/iso-oligos) offering, our [Custom cGMP oligos](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/custom-cgmp-oligos) come with consistency and the highest quality. Whether you want to commercialize or develop an assay around qPCR, isothermal amplification, NGS, or other technology, our products strike the right balance between breadth and depth. ## Scalable Small Interfering RNA (siRNA)[](https://www.sigmaaldrich.com) Our global manufacturing expertise ensures milligram and gram quantities of MISSION® *in viv*o quality siRNA, ideal for superior RNAi research in animals. Additionally, we produce other RNA types like miRNA and single-stranded RNA, all scalable to meet diverse research needs with excellence. [Request Quote (siRNA)](https://www.sigmaaldrich.com/US/en/services/support/mission-in-vivo-quality) * * * ### siRNA Product Benefits - Suitable for *in vivo* applications, including target validation and pre-clinical testing - Available for [MISSION Predesigned siRNA](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/gene-expression-and-silencing/mission-predesigned-sirna) or your custom siRNA sequences - Appropriate for direct delivery or as an essential component of your formulation strategy ## iScale Oligos siRNA Product Specifications[](https://www.sigmaaldrich.com) Custom scalable siRNA are available in a range of quantities, purifications and formats depending on your manufacturing needs. For anything marked as ‘Inquire’ below or if you have needs that are different from the other general specifications presented, please send a request to [sirnarequest@milliporesigma.com](mailto:sirnarequest@milliporesigma.com). Quantities•  3, 50 & 100 mg •  Inquire for larger quantities [Purification](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/dna-and-rna-purification/best-purification)• Desalt, RP-HPLC & *in vivo* Sequence Form• 19 to 50mer simplexes or duplexes [Modifications](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/qpcr/custom-dna-oligos-modifications)•  Biotin, phosphate, Amino-Modifier (C3, C6, C6 dT, C7, C12), 6-FAM™, Cyanine 3,    Cyanine 5, Cyanine 5.5, 2'-OMe & phosphorothioate •  Inquire for feasibility of Locked Nucleic Acid & other modifications [Quality Control](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/custom-dna-oligos-qc-qa)• 100% mass spectrometry* • Analytical HPLC available • Certificate of Analysis available Format• Supplied dry in tubes or plates • Pooling & aliquoting available (inquire for feasibility) Services• If the oligonucleotide is to be used *in vivo*, *in vivo* grade purification and these services   are recommended:   - Salt exchange (replaces toxic ammonium ions from the synthesis     with physiologically-tolerable sodium ions)   - Filtration (minimizes bacteria, molds, and other contaminants)   - Endotoxin testing (ensures gram-negative LPS is below an acceptable     threshold, otherwise the host may die from toxic shock as initiated by     an immune cascade) *\*Depending on manufacturing site, PAGE may be used to assess siRNA duplexes.* ### [](https://www.sigmaaldrich.com)__Demonstrated use of precision siRNA *in vivo*__ We teamed up with Bioo Scientific (now PerkinElmer) to validate our MISSION *in viv*o quality siRNA (see article in Drug Discovery & Development—Aug 1, 2008). In these experiments, H1155luc cells were injected into NOD/SCID mice. Tumors formed and were injected with luciferase-specific siRNA or non-targeting *in vivo* quality siRNA formulated in MaxSuppressor™ *in vivo* RNA-LANCEr II. After 24 hours, D-luciferin solution was injected, and the animals were imaged using the NightOWL II LB 983 instrument from Berthold Technologies. Animals treated with luciferase-specific siRNA showed significant reduction of luciferase activity while non-targeting siRNA-treated animals were positive for luminescence ([Figure 1](https://www.sigmaaldrich.com#figure1)). These data demonstrate the effectiveness of *in vivo* quality siRNA formulated in MaxSuppressor™ *in vivo* RNA-LANCEr II *in vivo* delivery agent. ![Photographs of fluorescently glowing, Luciferase-specific siRNA treated animals and non-glowing non-targeting siRNA treated control animal showing the effectiveness of in-vivo-quality siRNA in delivering luciferase](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/categories/custom-products/custom-oligo1.jpg "Luciferase-specific siRNA treated animals and non-targeting siRNA treated control animal") __Figure 1.__Luciferase-specific siRNA treated animal on the left; non-targeting siRNA treated animal on the right At 72 hours post-injection, the tumors were removed, and the protein was extracted and normalized using a Bradford assay. Luciferase concentrations were assayed using a Luciferase ELISA. Protein reduction is represented relative to a non-targeting RNAi agent treated animal. Luciferase activity was reduced by two-fold in the tumors (__Figure 2__). ![Bar chart showing the percent luciferase protein expression in tumors from three Luciferase-specific siRNA treated mice and one non-targeting siRNA mouse. Luciferase activity was reduced by two-fold in the tumors](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/categories/custom-products/custom-oligo2.gif "Luciferase activity") __Figure 2.__Percent luciferase protein expression in Luciferase-specific siRNA treated animals and controls measured by ELISA. Each replicate is a different mouse. [Request Quote (siRNA)](https://www.sigmaaldrich.com/US/en/services/support/mission-in-vivo-quality) If additional help is needed, please consult our technical services group at [oligotechserv@milliporesigma.com](mailto:oligotechserv@milliporesigma.com).  MISSION is a trademark of Merck KGaA, Darmstadt, Germany and/or its affiliates. [Label License](https://www.sigmaaldrich.com/US/en/life-science/legal/product-licenses/mission-rnai-licenses). ### References 1\. Stany MP, Vathipadiekal V, Ozbun L, Stone RL, Mok SC, Xue H, Kagami T, Wang Y, McAlpine JN, Bowtell D, et al. Identification of Novel Therapeutic Targets in Microdissected Clear Cell Ovarian Cancers. PLoS ONE. 6(7):e21121. [https://doi.org/10.1371/journal.pone.0021121](https://doi.org/10.1371/journal.pone.0021121) 2\. Beghin A, Belin S, Hage-Sleiman R, Brunet Manquat S, Goddard S, Tabone E, Jordheim LP, Treilleux I, Poupon M, Diaz J, et al. Correction: ADP Ribosylation Factor Like 2 (Arl2) Regulates Breast Tumor Aggressivity in Immunodeficient Mice. PLoS ONE. 4(11): [https://doi.org/10.1371/annotation/b0d43779-c9aa-44fe-a46d-71d7d2bc4a6a](https://doi.org/10.1371/annotation/b0d43779-c9aa-44fe-a46d-71d7d2bc4a6a) 3\. Ramachandran V, Arumugam T, Langley R, Hwang RF, Vivas-Mejia P, Sood AK, Lopez-Berestein G, Logsdon CD. The ADMR Receptor Mediates the Effects of Adrenomedullin on Pancreatic Cancer Cells and on Cells of the Tumor Microenvironment. PLoS ONE. 4(10):e7502. [https://doi.org/10.1371/journal.pone.0007502](https://doi.org/10.1371/journal.pone.0007502) 4\. Brahmamdam P, Watanabe E, Unsinger J, Chang KC, Schierding W, Hoekzema AS, Zhou TT, McDonough JS, Holemon H, Heidel JD, et al. 2009. TARGETED DELIVERY OF siRNA TO CELL DEATH PROTEINS IN SEPSIS. 32(2):131-139. [https://doi.org/10.1097/shk.0b013e318194bcee](https://doi.org/10.1097/shk.0b013e318194bcee) 5\. Fitamant J, Guenebeaud C, Coissieux M, Guix C, Treilleux I, Scoazec J, Bachelot T, Bernet A, Mehlen P. 2008. Netrin-1 expression confers a selective advantage for tumor cell survival in metastatic breast cancer. Proceedings of the National Academy of Sciences. 105(12):4850-4855. [https://doi.org/10.1073/pnas.0709810105](https://doi.org/10.1073/pnas.0709810105) 6\. Watabe M, Aoyama K, Nakaki T. 2008. A Dominant Role of GTRAP3-18 in Neuronal Glutathione Synthesis. Journal of Neuroscience. 28(38):9404-9413. [https://doi.org/10.1523/jneurosci.3351-08.2008](https://doi.org/10.1523/jneurosci.3351-08.2008) * * * __Related Articles__ - [Custom GMP-Grade Oligos](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/custom-cgmp-oligos) - [Long Oligos](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/custom-dna-long-oligos) - [Antisense Oligonucleotides](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/gene-expression-and-silencing/antisense-oligonucleotides) - [Custom Next-Gen Sequencing Oligos](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/next-gen-sequencing/custom-next-gen-sequencing-oligos) - [Extract-N-Amp™ Product Guide](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/pcr/extract-n-amp-product-guide) __Related Product Categories__ - [Custom DNA Oligos & qPCR Probes](https://www.sigmaaldrich.com/US/en/products/molecular-biology-and-functional-genomics/oligos-and-qpcr-probes/custom-predesigned-dna-oligos-and-qpcr-probes) - [Custom & Predesigned RNA Oligos, siRNA Duplexes and Controls](https://www.sigmaaldrich.com/US/en/products/molecular-biology-and-functional-genomics/oligos-and-qpcr-probes/custom-predesigned-rna-oligos-sirna-duplexes-controls) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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