[Skip to Content](https://www.sigmaaldrich.com#main-content) [![MilliporeSigma](https://www.sigmaaldrich.com/static/logos/purple/millipore_sigma.svg)](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Polymerase Chain Reaction Applications](https://www.sigmaaldrich.com/US/en/applications/genomics/pcr)OligoArchitect™ Consultative # OligoArchitect™ Consultative [Request Design](https://www.sigmaaldrich.com/US/en/services/support/probe-design) For more complex and demanding applications, utilize our consultative service to ensure the success of your assay. With personal consultation from our expert, technical support scientists, your request, including all sequences, will be MIQE compliant and returned to you within 24-48 hours. ### Specifications Design Modules__PCR, qPCR & dPCR Applications__ - SYBR®Green I Primers - Dual-Labeled Probes1 - Molecular Beacons2 - LightCycler® Probes3 - Traditional PCR - SNP detection - Allele discrimination - Pathogen detection - Multiplexing - Viral load quantification - Gene expression analysis - Gene copy determination - Endpoint genotyping - Methylation analysis - High-resolution melting analysis - Transcript detection across exon junctions - Haplotyping - Northern blotting - Southern blotting __Other Applications__ - FISH (fluorescence in situ hybridization) - ASO (antisense oligonucleotides)   __Modifications__ - Add Locked Nucleic Acid to any of the above design modules - Add MGB:EDQ (Minor Groove Binder:Eclipse Dark Quencher) to Dual-Labeled Probes 1 Also known as hydrolysis probes, which are used in the 5’ nuclease assay 2 Also known as hybridization probes 3 Also known as dual-hybridization probes Our consultative service uses Beacon Designer™ from Premier Biosoft International. Blastn and mfold are used to determine assay specificity. ## Materials Sorry, an unexpected error has occurred Response not successful: Received status code 500 __Related Articles__ - [Milli-Q® IQ 7000 Ultrapure Water System](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/milli-q-iq-7000) - [Milli-Q® IQ 7003/05/10/15 Ultrapure & Pure Lab Water Purification System](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/wet-chemical-analysis/milli-q-iq7003-7005) - [Custom Oligos for Commercial Use](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/iso-oligos) - [Custom LAMP Primers in Partnership with New England Biolabs](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/custom-lamp-primers) - [Easy Oligo](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/easy-oligo) - [Fast siRNA](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/fast-sirna-oligos) - [Scalable, High-Quality Custom Oligonucleotides for Commercial PCR, qPCR & NGS](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/iso-oligos) - [KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/kod-one-pcr-master-mix) - [View More](https://www.sigmaaldrich.com/US/en/search/facet-search?focus=sitecontent&term=facet-search) __Related Product Categories__ - [NGS Library Preparation](https://www.sigmaaldrich.com/US/en/products/molecular-biology-and-functional-genomics/ngs-library-prep) Top __Sign In To Continue__ To continue reading please sign in or create an account. Sign In__Don't Have An Account?__Register An unknown error has occured.