[Skip to Content](https://www.sigmaaldrich.com#main-content) [![MilliporeSigma](https://www.sigmaaldrich.com/static/logos/purple/millipore_sigma.svg)](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products ProductsApplicationsServicesResourcesSupport [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Polymerase Chain Reaction Applications](https://www.sigmaaldrich.com/US/en/applications/genomics/pcr)Custom DNA/RNA Oligos & qPCR Probes Resources # Custom DNA/RNA Oligos & qPCR Probes Resources For over 35 years we have been providing custom and predesigned oligos and qPCR probes for PCR, cloning, DNA sequencing, and gene expression. We offer tools for oligo and qPCR probe design including [OligoArchitect™](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/oligoarchitect-online) Primer and Probe Design Solutions and [OligoEvaluator™](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/oligo-evaluator-for-tm-calculation-primer-analysis): Oligonucleotide Sequence Calculator that provides primer analysis values for PCR and helps determine volumes for resuspension and dilution of your DNA oligos to desired final concentrations. ## How To Order Oligo Products Online Tutorial for Ordering Custom Oligo Products. Highlighting navigation to ordering, manual and multiple entry of DNA Oligos, DNA in Plates, qPCR Probes, siRNA and predesigned siRNA. * * * ## Featured Articles and Protocols - [OligoEvaluator™ for Tm Calculation & Primer Analysis](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/oligo-evaluator-for-tm-calculation-primer-analysis) We offer an easy-to-use Oligo Analyzer tool, OligoEvaluator™ that provides better primer dimer analysis values for PCR, and serves as an online oligonucleotide sequence calculator and Tm calculator. - [DNA Oligonucleotide Synthesis](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/dna-oligonucleotide-synthesis) Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis. - [Annealing Oligonucleotides Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/pcr/annealing-oligos) Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. * * * ## Technical Literature - [DNA Oligonucleotide Synthesis](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/dna-oligonucleotide-synthesis) Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis. - [Oligonucleotide Purification](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/dna-and-rna-purification/best-purification) Learn about the importance of oligonucleotide purification, recommended methods, common techniques and applications, and key steps in the processes. - [Oligonucleotide Quantification](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/quantitation-of-oligos) DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature. - [Phosphorothioate Oligonucleotides](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/gene-expression-and-silencing/phosphorothioates) Phosphorothioate is a widely used backbone variant effective in creating oligonucleotides for antisense knockdowns. - [Phosphorothioate Chromatography Profile](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/large-molecule-hplc/profile-analysis-of-phosphorothiolated-oligos) Understand the impact of phosphorothioate internucleotide linkage addition to oligonucleotide chromatography profiles. - [Choosing the Right Custom qPCR Probe](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/qpcr/choosing-the-right-probe) View a summary of the applications using commonly ordered custom qPCR probes. - [Dual-Labeled Probes in Digital PCR](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/dual-labeled-probes-for-digital-pcr) Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences. - [Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/locked-nucleic-acids-faq) Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications. - [BLACK HOLE QUENCHER™ (BHQ™)](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/qpcr/black-hole-quenchers) Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR. - [Oligonucleotide Handling & Stability](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/dna-and-rna-purification/oligonucleotide-handling-and-stability) Handing and stability techniques to reduce variation and ensure trouble-free experiments. - [Oligonucleotide Melting Temperature](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/pcr/oligos-melting-temp) Learn about methods for calculating oligonucleotide melting temperature (Tm). ## Protocols, Procedures & Quality - [Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/pcr/nhs-ester-oligonucleotide-conjugation) Protocol for conjugating NHS-ester modifications to an oligonucleotide with an amino label. - [Annealing Oligonucleotides Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/pcr/annealing-oligos) Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. - [Oligo Reduction](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/materials-science-and-engineering/drug-delivery/oligo-reduction) Follow this DDT reduction protocol to reduce disulfide bonds in thiol-modified oligonucleotides. - [Oligonucleotide Quality Control & Quality Assurance](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/custom-dna-oligos-qc-qa) The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification. - [ISO Certificates and Site Quality Self-Assessments](https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/iso-certificates) We are committed to product quality, manufacturing effectiveness and meeting customer expectations. * * * __Related Product Resources__ - [Oligo Product Reviews by SelectScience®](https://www.sigmaaldrich.com/US/en/technical-documents/product-supporting/oligos-qpcr-probes/oligo-product-reviews) * * * ## Videos Slide 1 of 5 [![MPower Payment Program](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/mpower-video-poster.jpg "MPower Payment Program")](https://www.sigmaaldrich.com) [MPower Payment Program](https://www.sigmaaldrich.com) Convenient payment program for online ordering of transactional Custom DNA & RNA products. Check for country availability. [![Online Oligo Ordering](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/oligo-ordering.jpg "Online Oligo Ordering")](https://www.sigmaaldrich.com) [Online Oligo Ordering](https://www.sigmaaldrich.com) Overview of benefits of ordering oligos [![Compliant Oligo Manufacturing](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/compliant-oligos.jpg "Compliant Oligo Manufacturing")](https://www.sigmaaldrich.com) [Compliant Oligo Manufacturing](https://www.sigmaaldrich.com) Video overview of our capabilities in oligo scale-up and manufacturing [![Next-Gen Sequencing Oligos ](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/next-gen-oligos.jpg "Next-Gen Sequencing Oligos ")](https://www.sigmaaldrich.com) [Next-Gen Sequencing Oligos](https://www.sigmaaldrich.com) Video overview of our next-gen sequencing oligos for manufacturing and index adapters [![Oligo Delivery ](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/oligo-delivery.jpg "Oligo Delivery ")](https://www.sigmaaldrich.com) [Oligo Delivery](https://www.sigmaaldrich.com) Secure supply chain, just-in-time quick delivery and a tradition of quality and reliability * * * ## Product Citations & Our Publications Custom DNA Oligos Custom qPCR Probes KiCqStart® Primers Our Publications 1\. Guo Y, Ye JY, Divin C, Huang B, Thomas TP, Baker, Jr. JR, Norris TB. 2010. Real-Time Biomolecular Binding Detection Using a Sensitive Photonic Crystal Biosensor. Anal. Chem.. 82(12):5211-5218. [https://doi.org/10.1021/ac100576y](https://doi.org/10.1021/ac100576y) 2\. Kudla G, Murray AW, Tollervey D, Plotkin JB. 2009. Coding-Sequence Determinants of Gene Expression in Escherichia coli. Science. 324(5924):255-258. [https://doi.org/10.1126/science.1170160](https://doi.org/10.1126/science.1170160) 3\. Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F. Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE. 4(12):e8291. [https://doi.org/10.1371/journal.pone.0008291](https://doi.org/10.1371/journal.pone.0008291) 4\. Omumi A, Beach DG, Baker M, Gabryelski W, Manderville RA. 2011. Postsynthetic Guanine Arylation of DNA by Suzuki?Miyaura Cross-Coupling. J. Am. Chem. Soc.. 133(1):42-50. [https://doi.org/10.1021/ja106158b](https://doi.org/10.1021/ja106158b) 5\. Pandey M, Syed S, Donmez I, Patel G, Ha T, Patel SS. 2009. Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature. 462(7275):940-943. [https://doi.org/10.1038/nature08611](https://doi.org/10.1038/nature08611) 6\. Wang Z, Elbaz J, Remacle F, Levine RD, Willner I. 2010. All-DNA finite-state automata with finite memory. Proceedings of the National Academy of Sciences. 107(51):21996-22001. [https://doi.org/10.1073/pnas.1015858107](https://doi.org/10.1073/pnas.1015858107) 7\. Yang L, Kemadjou JR, Zinsmeister C, Bauer M, Legradi J, Müller F, Pankratz M, Jäkel J, Strähle U. 2007. Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. Genome Biol. 8(10):R227. [https://doi.org/10.1186/gb-2007-8-10-r227](https://doi.org/10.1186/gb-2007-8-10-r227) 8\. Zykovich A, Korf I, Segal DJ. 2009. Bind-n-Seq: high-throughput analysis of in vitro protein?DNA interactions using massively parallel sequencing. 37(22):e151-e151. [https://doi.org/10.1093/nar/gkp802](https://doi.org/10.1093/nar/gkp802) 9\. Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T. 2011. Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2?-O-methyl RNA probes in living cells. 39(4):e20-e20. [https://doi.org/10.1093/nar/gkq1196](https://doi.org/10.1093/nar/gkq1196) 1\. Josefsen MH, Löfström C, Sommer HM, Hoorfar J. 2009. Diagnostic PCR: Comparative sensitivity of four probe chemistries. Molecular and Cellular Probes. 23(3-4):201-203. [https://doi.org/10.1016/j.mcp.2009.02.003](https://doi.org/10.1016/j.mcp.2009.02.003) 2\. Saito Y, Kitamura H, Hijikata A, Tomizawa-Murasawa M, Tanaka S, Takagi S, Uchida N, Suzuki N, Sone A, Najima Y, et al. 2010. Identification of Therapeutic Targets for Quiescent, Chemotherapy-Resistant Human Leukemia Stem Cells. Science Translational Medicine. 2(17):17ra9-17ra9. [https://doi.org/10.1126/scitranslmed.3000349](https://doi.org/10.1126/scitranslmed.3000349) 1\. Schutkowski A, König B, Kluge H, Hirche F, Henze A, Schwerdtle T, Lorkowski S, Dawczynski C, Gabel A, Große I, et al. 2019. Metabolic footprint and intestinal microbial changes in response to dietary proteins in a pig model. The Journal of Nutritional Biochemistry. 67149-160. [https://doi.org/10.1016/j.jnutbio.2019.02.004](https://doi.org/10.1016/j.jnutbio.2019.02.004) 2\. Schutkowski A, Max D, Bönn M, Brandsch C, Grundmann SM, Hirche F, Staege MS, Stangl GI. 2018. Vitamin D Does Not Play a Functional Role in Adipose Tissue Development in Rodent Models. Mol. Nutr. Food Res.. 62(4):1700726. [https://doi.org/10.1002/mnfr.201700726](https://doi.org/10.1002/mnfr.201700726) 3\. Kühne H, Hause G, Grundmann SM, Schutkowski A, Brandsch C, Stangl GI. 2016. Vitamin D receptor knockout mice exhibit elongated intestinal microvilli and increased ezrin expression. Nutrition Research. 36(2):184-192. [https://doi.org/10.1016/j.nutres.2015.10.005](https://doi.org/10.1016/j.nutres.2015.10.005) 4\. Schutkowski A, Wege N, Stangl GI, König B. Tissue-Specific Expression of Monocarboxylate Transporters during Fasting in Mice. PLoS ONE. 9(11):e112118. [https://doi.org/10.1371/journal.pone.0112118](https://doi.org/10.1371/journal.pone.0112118) 5\. Kühne H, Schutkowski A, Weinholz S, Cordes C, Schierhorn A, Schulz K, König B, Stangl GI. 2014. Vitamin D receptor regulates intestinal proteins involved in cell proliferation, migration and stress response. Lipids Health Dis. 13(1):51. [https://doi.org/10.1186/1476-511x-13-51](https://doi.org/10.1186/1476-511x-13-51) 1\. Hudnall SD, Chen T, Allison P, Tyring SK, Heath A. 2008. Herpesvirus prevalence and viral load in healthy blood donors by quantitative real-time polymerase chain reaction. Transfusion. 48(6):1180-1187. [https://doi.org/10.1111/j.1537-2995.2008.01685.x](https://doi.org/10.1111/j.1537-2995.2008.01685.x) \*qPCR primers and probes were used to detect virus in blood samples; a Custom Products scientist co-authored this paper. __Tutorials & Tools__ - [Custom Oligos & qPCR Probes Online Tools](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/custom-oligos-and-qpcr-probes-online-tools) __Publications & Case Studies__ - [Antisense Oligonucleotides](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/gene-expression-and-silencing/antisense-oligonucleotides) __Licenses & FAQs__ - [Oligonucleotide Label Licenses](https://www.sigmaaldrich.com/US/en/life-science/legal/product-licenses/oligonucleotide-label-licenses) - [Oligonucleotide FAQ](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/pcr/oligos-faqs) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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