[Skip to Content](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products ProductsApplicationsServicesResourcesSupport [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Pathogen & Spoilage Testing](https://www.sigmaaldrich.com/US/en/applications/microbiological-testing/pathogen-and-spoilage-testing)HybriScan® Rapid Microbial Test System # HybriScan® Rapid Microbial Test System The routine control of microbial contamination in food production, from raw materials to finished goods, conventional standard cultivation-based methods are time consuming and often take from 2 to 15 days to get results. Our HybriScan® assays provides fast and accurate molecular detection of spoilage organisms and pathogens, without the need to use expensive PCR methods. HybriScan® Molecular Screening System is suitable for the safety and quality control of beverages, water, and food. - [Rapid Microbiological Method: Sandwich Hybridization](https://www.sigmaaldrich.com#rapid) - [Sensitivity, Specificity and Flexibility of Sandwich Hybridization](https://www.sigmaaldrich.com#sandwich) - [Advantages Of HybriScan® Technology Over Other Microbial Detection Techniques](https://www.sigmaaldrich.com#advantages) - [HybriScan® Wastewater Bacterial Count Calculator](https://www.sigmaaldrich.com#wastewater) - [HybriScan®D (Detection) Kits](https://www.sigmaaldrich.com#kits) - [HybriScan®I (Identification) Kits](https://www.sigmaaldrich.com#identification) [Speak to a specialist](https://www.sigmaaldrich.com/US/en/campaigns/microbial-identification-tests) ## [](https://www.sigmaaldrich.com)Rapid Microbiological Method: Sandwich Hybridization The HybriScan® system is based on the detection of microbe-specific rRNA using sandwich hybridization. The method simply needs a centrifuge, a thermal mixer, and an optional microplate reader. The complete analysis can be achieved in 2 hours for the HybriScan®*D* kit (quantitative kits) and about 1 hour for the HybriScan®*I* kit (identification kits). Qualitative results can be determined by eye or, for quantitative measurement, used with a microplate reader.  __Figure 1.__HybriScan® system screening workflow with an analysis time of approx. 2–2.5 hours. [Download HybriScan® Brochure](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/392/br3818en-ms.pdf) ## [](https://www.sigmaaldrich.com)Sensitivity, Specificity and Flexibility of Sandwich Hybridization Sandwich hybridization is very sensitive and can detect attomoles of the target rRNA molecules in yeast or bacteria.1-3 These types of cells comprise a large number of rRNA-containing ribosomes; a single cell therefore contains several thousand copies of rRNA but only one DNA copy. Sandwich hybridization provides sensitivity in crude biological samples because it is not susceptible to matrix interference. By using specific probes, the HybriScan® system allows both genus or species-specific detection and it is therefore applicable to many analytical fields, including monitoring the microbial content of beer, wine, non-alcoholic beverages, drinking water, a wide variety of foods and wastewater.  __Figure 2.__HybriScan® technology - Detects viable microorganisms only—minimizes false-positive results from dead bacteria - Not sensitive to sample matrix - Genus- and species-specific detection - Results in just 2 hours after enrichment - Broad application range: beer, wine, non-alcoholic beverages, drinking water, food and wastewater - Can be used to detect non-culturable microbes ### Advantages of HybriScan® technology over other microbial detection techniques Detection TechnologyAdvantageDisadvantage HybriScan® system - Detects only living cells - Minimal interference by sample matrix - High specificity - Low cross-reactivity - Easy handling - Cost-efficient read-out - Quantitative and qualitative - High sample throughput (96-microwell plates) - Detects of non-culturable microbes - No differentiation of serotypes or subspecies - Limited probe design (rRNA target) PCR - High sample throughput - Sensitive - Quantitative - No live/dead cell differentiation - Sensitive to matrix interference (high extraction effort) - Susceptible to polymerase inhibition ELISA - Differentiation of serotypes or subspecies - High sample throughput (96-microwell plates) - Quantitative and qualitative - Low sensitivity - Low specificity, higher cross-reactivity - Slow and expensive assay development Conventional cultivation-based methods - Relatively inexpensive - Simple - Specific - Widely accepted method - Time-consuming (up to 10 days) - No detection of non-culturable microbes - Low sample throughput - Laborious ## [](https://www.sigmaaldrich.com)HybriScan® Wastewater Bacterial Count Calculator For our Wastewater Kits - ([HybriScan®*D* Waste Water Microthrix parvicella](https://www.sigmaaldrich.com/US/en/product/SIAL/04447) and  [HybriScan®*D* Waste Water Total Bacterial Count](https://www.sigmaaldrich.com/US/en/product/sial/78436)), we are providing an excel sheet (below) for calculation of total bacterial count, Microthrix count, and the ratio of Microthrix: Total Bacterial Count. ## HybriScan®*D* (Detection) Kits Sorry, an unexpected error has occurred Response not successful: Received status code 500 ## HybriScan® *I* (Identification) Kits Sorry, an unexpected error has occurred Response not successful: Received status code 500 ### References 1\. Huhtamella S, Leinonen M, Nieminen T, Fahnert B, Myllykoski L, Breitenstein A, Neubauer P. 2007. RNA-based sandwich hybridisation method for detection of lactic acid bacteria in brewery samples. Journal of Microbiological Methods. 68(3):543-553. [https://doi.org/10.1016/j.mimet.2006.10.009](https://doi.org/10.1016/j.mimet.2006.10.009) 2\. Leskelä T, Tilsala-Timisjärvi A, Kusnetsov J, Neubauer P, Breitenstein A. 2005. Sensitive genus-specific detection of Legionella by a 16S rRNA based sandwich hybridization assay. Journal of Microbiological Methods. 62(2):167-179. [https://doi.org/10.1016/j.mimet.2005.02.008](https://doi.org/10.1016/j.mimet.2005.02.008) 3\. Rautio J, Barken K, Lahdenperä J, Breitenstein A, Molin S, Neubauer P. 2003. Microb Cell Fact. 2(1):4. [https://doi.org/10.1186/1475-2859-2-4](https://doi.org/10.1186/1475-2859-2-4) __Related Technical Articles__ - [Media Fills Test for Beverages](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/microbiological-testing/media-fills/linden-grain) - [*Lactobacilli* Basics, Testing & Identification](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/clinical-testing-and-diagnostics-manufacturing/bacteriology/lactobacilli) - [Microbial Detection Using Chromogenic and Fluorogenic Culture Media](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/clinical-testing-and-diagnostics-manufacturing/bacteriology/overview-chromogenic-media) - [Identification of Microorganisms Based on Color](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/clinical-testing-and-diagnostics-manufacturing/cytology-and-microscopy/color-identification-microorganisms) - [Culture Method for Beer Quality Control](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/culture-method-beer-quality) - [Detection and Differentiation of Staphylococcus Aureus on Baird Parker Agar](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/detection-and-differentiation) - [Differentiation of Escherichia coli from coliforms](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/differentiation-of-e-coli-coliforms) - [DiluCult™ Gravimetric Dilutor & Sample Homogenizer](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/food-and-beverage-testing-and-manufacturing/microbiological-analysis-for-food-and-beverage/dilucult-gravimetric-dilutor-homogenizer) - [View More](https://www.sigmaaldrich.com/US/en/search/facet-search?focus=sitecontent&term=facet-search) __Related Product Categories__ - [Microbial Identification Tests](https://www.sigmaaldrich.com/US/en/products/industrial-microbiology/microbial-identification-tests) - [Microbial Culture Media](https://www.sigmaaldrich.com/US/en/products/industrial-microbiology/microbial-culture-media) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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