Life Science

Lentiviral Packaging

The goal of this exercise is to learn lentiviral packaging. For this example we will be using the HEK 293T cell line. We will perform two separate transfection reactions with the goal of producing functional viral particles. First, we will transfect cells with a combination of packaging plasmid mix and TurboGFP control vector. The second transfection reaction will contain packaging plasmid mix and Empty control vector. The transfected HEK 293T cells will be monitored for TurboGFP expression, which is indicative of successful viral production.