Live Cell Imaging of Apoptosis in Immune Cells using the CellASIC® ONIX2 Microfluidic System

Jurkat cells (88042803) were first loaded onto the CellASIC ONIX M04T Pad Trap Plate (M04T-01-5PK) and were then incubated with BioTracker NucView® 488 Green Caspase-3 Dye (SCT101) and Annexin V Texas Red (Red) for the first 2 hours in complete RPMI-1640 media (SLM-240-B), followed by treatment of 2.5 µg/mL CD-95 antibody (05-201)  (experiment group) or no treatment (control) for 8 hours. Time-lapse imaging was taken on cells at a 10-minute interval under 400x magnification using a FITC and Texas-Red filter.

Ordering Info

Description Part Number
BioTracker NucView® 488 Green Caspase-3 Dye (PBS) SCT101
CellASIC® ONIX Pad Trap Plate (4-chamber, trap heights 12 micron), 5pk M04T-01-5PK
Jurkat E6.1 Cell Line human 88042803
RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS SLM-240-B
Anti-Fas Antibody (human, activating), clone CH11 05-201