Spoilage and Sterility Tests in Beverages: New Revised IFU Method No. 12 for Detection of Alicyclobacillus in Fruit Juices, and Sterility Testing of Aseptic Filling Lines


Alicyclobacillus spp. can be difficult to control in fruit juice products as their spores survive juice pasteurization temperatures and may germinate and grow after processing if conditions are suitable. The economic impact of such incidents can be very high. The International Fruit and Vegetable Juice Association (IFU) Microbiological Working Group has revised, improved and validated their worldwide accepted method for the detection of Alicyclobacillus spp. in fruit juices with improved culture media formulations including performance criteria that agree with ISO procedures.

For aseptic validation of filling lines, Sidel microbiological validations are carried out in two subsequent phases. The first takes place on Sidel premises to ensure the decontamination efficiency of the sterilization applied to packaging, while the second is carried out on the complete line installed at the customer site to ensure the commercial sterility of the filled product. Depending on the client’s product, different microbiological broths for low acid (pH > 4.6) or high acid (pH ≤ 4.6) applications are useful.

Areas Covered in the Webinar

This webinar explains the new revised IFU Method No. 12 from 2018 for detection of Alicyclobacillus spp. in fruit juices and provides information about validation of aseptic filling lines in beverage production.



Dr.-Ing. Andreas Politzer
Senior Manager Microbiology and Food Safety
ADM WILD Europe GmbH & Co. KG
Eppelheim, Germany





Dr. Silvia Garulli
Microbiology Laboratory Manager
Sidel Parma, Italy





Dr. Andreas Bubert
Senior Product Manager Culture Media Food & Beverage
Merck KGaA, Darmstadt, Germany





Barbara Gerten
Senior Scientist Traditional Microbiology
Merck KGaA, Darmstadt, Germany