If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
S4521
Saponin from Quillaja sp.
Sapogenin content 20-35 %
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$69.70
$141.00
$69.70
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description
non-ionic
Quality Level
composition
Sapogenin content, 20-35%
technique(s)
HPLC: suitable
protein quantification: suitable
sulfated ash
≤10%
solubility
soluble (Water solubility: 2.000 g/l at 19,5 °C - OECD Test Guideline 105 - completely)
SMILES string
O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O[C@H]2[C@@H](O[C@@H]([C@H]([C@@H]2O)O[C@@H]8O[C@@H]([C@H]([C@@H]([C@H]8O)O)O)CO)C(=O)O)O[C@@H]3[C@@](C4[C@@](C5[C@]([C@@]6(C[C@H]([C@]7([C@H]([C@@H](C(CC7C6=CC5)(C)C)OC(=O)\C(=C/C)\C)OC(=O)C)CO)O)C)(CC4)C)(CC3)C
InChI
1S/C55H86O24/c1-10-23(2)46(71)79-43-44(72-24(3)60)55(22-59)26(17-50(43,4)5)25-11-12-30-51(6)15-14-32(52(7,21-58)29(51)13-16-53(30,8)54(25,9)18-31(55)61)75-49-41(77-48-38(67)36(65)34(63)28(20-57)74-48)39(68)40(42(78-49)45(69)70)76-47-37(66)35(64)33(62)27(1
InChI key
AXNVHPCVMSNXNP-ZELRDNAQSA-N
1 of 4
This Item | S7900 | SAE0073 | 47036 |
|---|---|---|---|
| technique(s) HPLC: suitable, protein quantification: suitable | technique(s) HPLC: suitable, protein quantification: suitable | technique(s) HPLC: suitable, LC/MS: suitable, protein quantification: suitable | technique(s) HPLC: suitable, protein quantification: suitable |
| description non-ionic | description non-ionic | description non-ionic | description - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Sapogenin content, 20-35% | composition Sapogenin content, ≥10% | composition Sapogenin content, 20-35% | composition Sapogenin, 8-25% |
| sulfated ash ≤10% | sulfated ash <20% | sulfated ash ≤10% | sulfated ash - |
General description
Application
Biochem/physiol Actions
Preparation Note
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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How can I determine the shelf life / expiration / retest date of this product?
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Is this product suitable for downstream molecular tests if used in a lysis buffer for DNA extraction ?
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This product has not been tested for suitability with any molecular tests after being used in a DNA lysis buffer. Validation by the intended user is necessary, as this has not been assessed. Researchers associated with Radboud University Medical Center have reported the usage of product 47036, or a molecular biology grade saponin, for bacterial DNA isolation prior to qPCR. For more details, refer to the citation below.
Optimized DNA isolation method for microbiome analysis of human tissues
Carlijn Bruggeling, Daniel R. Garza, Soumia Achouiti, Wouter Mes, Bas E. Dutilh, Annemarie Boleij
bioRxiv 2020.08.25.267641; doi: https://doi.org/10.1101/2020.08.25.267641Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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