MAB2223
Anti-DNP Antibody, clone 9H8.1
clone 9H8.1, from mouse
Synonym(s):
Dinitrophenol
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
clone
9H8.1, monoclonal
species reactivity (predicted by homology)
all
technique(s)
western blot: suitable
isotype
IgG1κ
shipped in
wet ice
target post-translational modification
unmodified
1 of 4
This Item | MABE338 | MABC1236 | MABN448 |
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified antibody | antibody form purified immunoglobulin | antibody form purified antibody |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone 9H8.1, monoclonal | clone 7H8.1, monoclonal | clone 3A9.1, monoclonal | clone 1H8.1, monoclonal |
| isotype IgG1κ | isotype IgG2aκ | isotype IgG1κ | isotype IgG2aκ |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) immunohistochemistry: suitable, western blot: suitable |
General description
Dinitrophenol (DNP) is a small water-soluble, hydrophobic ligand that binds to transthyretin at acidic pH and also reduces the formation of amyloid fibril. DNP levels of 100 mg/l or more is greatly toxic to acclimated bacteria. DNP has been associated with possible therapies for neurodegenerative and neurological disorders.
Various kDa observed. The Oxyblot protein standards (S7151) were used to test this antibody. These standards are phosphorylase B, BSA, OVA, Carbonic Anhydrase, and trypsin inhibitor which all contain 1-3 DNP residues per protein.
Immunogen
KLH-conjugated DNP
Application
This Anti-DNP Antibody, clone 9H8.1 is validated for use in WB for the detection of DNP.
Biochem/physiol Actions
Demonstrated to react with a DNP moiety in a wider range of species. This antibody′s reactivity is expected to be species independent.
Physical form
Format: Purified
Analysis Note
Control
Oxyblot protein standards
Oxyblot protein standards
Evaluated by Western Blot in Oxyblot protein standards.
Western Blot Analysis: 0.5 µg/mL of this antibody detected DNP on 10 µg of Oxyblot protein standards.
Western Blot Analysis: 0.5 µg/mL of this antibody detected DNP on 10 µg of Oxyblot protein standards.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Jennifer B M Steffen et al.
The Journal of experimental biology, 226(15) (2023-07-20)
Coastal environments commonly experience fluctuations in salinity and hypoxia-reoxygenation (H/R) stress that can negatively affect mitochondrial functions of marine organisms. Although intertidal bivalves are adapted to these conditions, the mechanisms that sustain mitochondrial integrity and function are not well understood.
Linda Adzigbli et al.
Scientific reports, 14(1), 9658-9658 (2024-04-27)
Sessile benthic organisms like oysters inhabit the intertidal zone, subject to alternating hypoxia and reoxygenation (H/R) episodes during tidal movements, impacting respiratory chain activities and metabolome compositions. We investigated the effects of constant severe hypoxia (90 min at ~ 0% O2 ) followed
Lona Zeneyedpour et al.
International journal of molecular sciences, 23(9) (2022-05-15)
We investigated the feasibility of detecting the presence of specific autoantibodies against potential tumor-associated peptide antigens by enriching these antibody-peptide complexes using Melon Gel resin and mass spectrometry. Our goal was to find tumor-associated phospho-sites that trigger immunoreactions and raise
Jennifer B M Steffen et al.
The Journal of experimental biology, 224(21) (2021-10-27)
Oxygen fluctuations are common in marine waters, and hypoxia-reoxygenation (H-R) stress can negatively affect mitochondrial metabolism. The long-lived ocean quahog, Arctica islandica, is known for its hypoxia tolerance associated with metabolic rate depression, yet the mechanisms that sustain mitochondrial function
Rita Araújo et al.
Bone, 158, 116355-116355 (2022-02-14)
Diabetes mellitus (DM) embrace a group of chronic metabolic conditions with a high morbidity, causing deleterious effects in different tissues and organs, including bone. Hyperglycemia seems to be one of the most contributing etiological factors of bone-related alterations, altering metabolic
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