91230

Trichloroacetic acid

ACS reagent grade, ≥99.5% (T), flakes, for the determination of Fe in blood according to Heilmeyer

• Synonym(s): TCA
• Linear Formula: Cl₃CCOOH
• CAS Number: 76-03-9
• Molecular Weight: 163.39
• NACRES: NA.21
• PubChem Substance ID: 57653429
• UNSPSC Code: 12352100
• EC Number: 200-927-2
• MDL number: MFCD00004177
• Beilstein/REAXYS Number: 970119

Properties

• Product Name: Trichloroacetic acid, ACS reagent, for the determination of Fe in blood according to Heilmeyer, ≥99.5%
• InChI key: YNJBWRMUSHSURL-UHFFFAOYSA-N
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• SMILES string: OC(=O)C(Cl)(Cl)Cl
• grade: ACS reagent
• vapor density: <1 (vs air)
• vapor pressure: 1 mmHg ( 51 °C)
• assay: ≥99.5% (T); ≥99.5%
• form: flakes
• quality: for the determination of Fe in blood according to Heilmeyer
• ign. residue: ≤0.03% (as SO₄)
• refractive index: n20/D 1.62 (lit.)
• bp: 196 °C (lit.)
• mp: 54-58 °C (lit.)
• solubility: water: soluble 81.7 g/L at 20 °C
• density: 1.62 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤10 mg/kg; nitrate (NO₃^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤200 mg/kg
• cation traces: Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• functional group: carboxylic acid; chloro
• Quality Level: 200

Description

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Trichloroacetic acid may be used in the following studies:

• Estimation of phosphorus in various biological samples (serum, urine and stool ash solutions).[1]
• Elimination of acid-soluble and lipid phosphorous during the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues.[2]
• Preparation of 2-methyl-4-nitroanilinium trichloroacetate trichloroacetic acid (2M4NATCA) crystals.[3]

10% Trichloroacetic acid may be employed for the preparation of radioactive proteins.[4]

General description

Trichloroacetic acid (TCA) is a chlorinated acetic acid. It is one of the major metabolites of trichloroethylene (TCE).[5]

Other Notes

Protein denaturant.

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H314,H410
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• wgk: WGK 2
• flash_point_f: >235.4 °F - closed cup
• flash_point_c: > 113 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges