STS0204

ECO TWEEN^® 80

viscous liquid

• Synonym(s): TWEEN^® 80, POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80
• CAS Number: 9005-65-6
• MDL number: MFCD00082107
• UNSPSC Code: 12161902
• NACRES: NA.28

Properties

• description: non-ionic
• Quality Level: 200
• Assay: ≥58%
• form: viscous liquid
• mol wt: micellar avg mol wt 79.000; average mol wt 1310
• composition: Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)
• greener alternative product characteristics: Use of Renewable Feedstocks; Design for Degradation; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• CMC: 0.012 mM (20-25°C)
• viscosity: 300—500 cP(25 °C)
• transition temp: cloud point 65 °C
• solubility: water: 100 mg/mL, clear to slightly hazy, faintly yellow
• density: 1.064 g/cm³
• HLB: 15.0
• greener alternative category: Aligned,
• SMILES string: CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO
• InChI: 1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+
• InChI key: RGPBUVUVZKQNHD-MDZDMXLPSA-N

Description

General description

ECO Tween^® 80, also known as Polyoxyethylenesorbitan monooleate, is a bio-based, nonionic detergent manufactured from a natural fatty acid. This surfactant, derived from natural sources, provides various functional advantages, such as detergency, emulsification, and wetting. Its versatility makes it well-suited for a variety of applications in biochemical and biological research, with common uses including cell lysis, protein extraction, nuclei isolation and cell fractionation.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is a biobased surfactant and is aligned with the 7th principle of Green Chemistry "Use of Renewable Feedstocks" and the 10th principle "Design for Degradation".

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

TWEEN^® 80 has been used in drug vehicle solution for specific injections in mice and rats.[1][2] It has been used for the determination of esterase activity in bacterial cells.[3]

Biochem/physiol Actions

TWEEN^® 80 is a polysorbate surfactant which helps in stabilizing proteins and in protein membrane studies. It is regularly used for the extraction of membrane proteins and isolation of nuclei from cells.[4][5][6]

Features and Benefits

• 100 % Renewable
• 100 % Bio-based
• Certified to the USDA BioPreferred Program
• Lower carbon footprint than petrochemical-based versions

• High-purity chemical suitable for a wide variety of research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

ECO TWEEN is a registered trademark of Croda Inc.

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): >235.4 °F - closed cup
• Flash Point(C): > 113 °C - closed cup