B3295

Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester

Name: Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester
Brand: SIGMA
Price: 134 USD

≥95% (TLC), powder

Synonym(s):

6-(Biotinamidocaproylamido)caproic acid N-hydroxysuccinimide ester, Biotin-X-X-NHS, N-Succinimidyl N-[6-(biotinylamino)caproyl]-6-aminocaproate

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About This Item

Empirical Formula (Hill Notation):

C₂₆H₄₁N₅O₇S

CAS Number:

89889-52-1

Molecular Weight:

567.70

UNSPSC Code:

12352106

NACRES:

NC.07

PubChem Substance ID:

24891708

MDL number:

MFCD00467154

Beilstein/REAXYS Number:

8377428

Key Documents

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Product Name

Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester, ≥95% (TLC), powder

InChI

1S/C26H41N5O7S/c32-20(27-16-8-2-4-12-24(36)38-31-22(34)13-14-23(31)35)10-3-1-7-15-28-21(33)11-6-5-9-19-25-18(17-39-19)29-26(37)30-25/h18-19,25H,1-17H2,(H,27,32)(H,28,33)(H2,29,30,37)/t18-,19-,25-/m0/s1

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCCCCC(=O)NCCCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2

InChI key

ATYCFNRXENKXSE-MHPIHPPYSA-N

assay

≥95% (TLC)

form

powder

solubility

DMF: 25 mg/mL

storage temp.

−20°C

Quality Level

200

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This Item	B3770	B2643	H1759
Sigma-Aldrich B3295 Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester	Sigma-Aldrich B3770 (+)-Biotinamidohexanoic acid hydrazide	Sigma-Aldrich B2643 Biotinamidohexanoic acid N-hydroxysuccinimide ester	Sigma-Aldrich H1759 (+)-Biotin N-hydroxysuccinimide ester
assay ≥95% (TLC)	assay ≥90% (TLC)	assay ≥98% (TLC)	assay ≥98% (HPLC)
solubility DMF: 25 mg/mL	solubility DMSO: 25 mg/mL	solubility H₂O: ≤2 mg/mL (with sonication), DMF: 50 mg/mL (Stable up to 2 months in dry DMF.)	solubility DMF: ≤50 mg/mL (Stable for at least one month in dry DMF), DMSO: soluble
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
form powder	form powder	form powder	form powder

Application

Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester has been used in human IgM biotinylation.[1]

Biotinylation reagent incorporating an extended 14-atom spacer can reduce steric hindrance in binding avidin or streptavidin to some biotinylated compounds and increase sensitivity for detection. Typically coupled to primary amine in the pH range 6.5-8.5.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Preparation of modified tubulins.

A Hyman et al.

Methods in enzymology, 196, 478-485 (1991-01-01)

Solid?phase preparation of protein complexes.

Pengo P, et al.

Journal of Molecular Recognition, 23(6), 551-558 (2010)

Ligand-based histochemical localization and capture of cells expressing heat-stable enterotoxin receptors.

J S Almenoff et al.

Molecular microbiology, 8(5), 865-873 (1993-05-01)

The heat stable enterotoxins (ST) of enterotoxigenic Escherichia coli (ETEC) cause diarrhoea by binding specific intestinal receptors. Precise histochemical localization of ST receptors could provide more information about the pathophysiology of secretory diarrhoea and the role of ST receptors in

Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin.

Aurore Cabanettes et al.

Angewandte Chemie (International ed. in English), 57(32), 10178-10181 (2018-06-30)

The mini fungal lectin PhoSL was recombinantly produced and characterized. Despite a length of only 40 amino acids, PhoSL exclusively recognizes N-glycans with α1,6-linked fucose. Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is

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Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester