C5405

Chicken Serum

Name: Chicken Serum
Brand: SIGMA

USA origin, sterile-filtered, suitable for cell culture

Synonym(s):

chicken sera

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.71

Biological source:

chicken

Origin:

USA origin

Sterility:

sterile-filtered

Form:

liquid

Shipped in:

dry ice

Key Documents

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biological source

chicken

Quality Level

500

sterility

sterile-filtered

form

liquid

composition

hemoglobin, ≤60 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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This Item	S2263	12138C	12731C
Sigma-Aldrich C5405 Chicken Serum	Sigma-Aldrich S2263 Sheep Serum	Sigma-Aldrich 12138C Bovine Calf Serum	Sigma-Aldrich 12731C Porcine Serum
biological source chicken	biological source sheep	biological source bovine (calf) (formula-fed)	biological source Porcine
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
origin USA origin	origin USA origin	origin USA origin	origin USA origin
form liquid	form -	form -	form -
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

Application

Chicken Serum has been used:

for pellet resuspension following centrifugation, to prepare zymosan suspension for respiratory burst assay[1]
as a supplement to Dulbecco′s modified Eagle′s medium (DMEM) to culture HeLa P cells[2]
for blocking in immunofluorescence (IF) double-staining[3]

Chicken serum is used in parasitology to study excystation, growth, survival and egg production of the microphallid trematode Maritrema novaezealandensis. Chicken serum is used for the serial cultivation of chicken keratinocytes.

Biochem/physiol Actions

Animal sera serve as a cost-effective alternative to basal cell culture media supplement. They are commonly used in immunodetection protocols such as immunohistochemistry (IHC), immunocytochemistry (ICC), and western blot to block nonspecific binding. Chicken serum is suitable for use in parasitology to study excystation, growth, survival, and egg production of the microphallid trematode Maritrema novaezealandensis.[4] It is used for the serial cultivation of chicken keratinocytes.[5]

Analysis Note

Endotoxin tested

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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A metabolic operon in extraintestinal pathogenic Escherichia coli promotes fitness under stressful conditions and invasion of eukaryotic cells.

Géraldine Rouquet et al.

Journal of bacteriology, 191(13), 4427-4440 (2009-04-21)

We identified a carbohydrate metabolic operon (frz) that is highly associated with extraintestinal pathogenic Escherichia coli (ExPEC) strains. The frz operon codes for three subunits of a phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) transporter of the fructose subfamily, for a transcriptional activator

In vitro cultivation of Maritrema novaezealandensis (Microphallidae): the effect of culture medium on excystation, survival and egg production.

B L Fredensborg et al.

Parasitology research, 95(5), 310-313 (2005-02-01)

In this study, the effect of the composition of culture medium on excystation, growth, survival and egg production was investigated for the recently discovered microphallid trematode Maritrema novaezealandensis. Metacercariae from the two second intermediate crab hosts, Macrophthalmus hirtipes and Halicarcinus

Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts.

Jeong Hyo Lee et al.

BMC molecular and cell biology, 21(1), 40-40 (2020-05-31)

In the poultry and livestock industries, precise genetic information is crucial for improving economic traits. Thus, functional genomic studies help to generate faster, healthier, and more efficient animal production. Chicken myoblast cells, which are required for muscle development and regeneration

Serial cultivation of chicken keratinocytes, a composite cell type that accumulates lipids and synthesizes a novel beta-keratin.

Amandine Vanhoutteghem et al.

Differentiation; research in biological diversity, 72(4), 123-137 (2004-05-26)

The epidermis of birds differs from that of mammals by the presence of intracellular lipid droplets and the absence of sebaceous glands. We describe here the cultivation of chicken epidermal keratinocytes; these cells cannot be grown in medium supplemented with

The nonconventional MHC class II molecule DM governs diabetes susceptibility in NOD mice.

Marc A J Morgan et al.

PloS one, 8(2), e56738-e56738 (2013-02-19)

The spontaneous destruction of insulin producing pancreatic beta cells in non-obese diabetic (NOD) mice provides a valuable model of type 1 diabetes. As in humans, disease susceptibility is controlled by the classical MHC class II genes that guide CD4(+) T

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