Each vial contains 100 ug each of the 9 constituent proteins, in lyophilized form. The recommended solvent is 0.1% trifluoroacetic acid (TFA) in water. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/421/524/msrt2bul.pdf
MSRT2
SigmaProt Intact Protein LC-MS Standard
Proteomics Retention Time Standard
Synonym(s):
Intact Protein LC-MS Standard, SigmaProtein Standard
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1 VIAL
$213.00
5 X 1 VIALS
$867.00
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This Item | P5369 | P0914 | P0834 |
|---|---|---|---|
| form ready-to-use solution | form - | form liquid | form liquid |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
The RP-LC-MS Protein Standard is a LC-MS platform standard that is intended to test LC-MS system suitability and intact protein mass measurement. The proteins in MSRT2 are chosen to represent an elution profile for complex proteomic samples. The proteins have been selected based on UV and electrospray response, and are readily observed by both modes of detection. After reconstitution, MSRT2 is an injection-ready standard. MSRT2 can be utilized in studies such as in top-down proteomics.
Other Notes
MSRT2 contains these 9 proteins:
- Ribonuclease B
- Insulin
- Lysozyme
- Transferrin
- Bovine serum albumin (BSA)
- Trypsin inhibitor
- β-lactoglobulin A
- Carbonic anhydrase
- Lactate dehydrogenase
used together
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Raffeal Bennett et al.
Journal of chromatography. A, 1523, 257-264 (2017-08-02)
Enhanced-fluidity liquid chromatography (EFLC) methods using methanol/H
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