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P1415

Prehybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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50 mL
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$251.00
5 x 50 mL
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$1,010.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

$251.00

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Quality Segment

form

liquid (2x concentrate)

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

-10 to -25°C

General description

Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.

Application

Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.

Preparation Note

Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
  • 200 μg/mL DNA
  • 2mg Bovine Serum Albumin (BSA)
  • 2mg Ficoll
  • 2mg Polyvinylpyrrolidone (PVP)
  • 0.15 M sodium citrate
  • 1.5M NaCl

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This Item
H7140S2404S7899
description

for Northern and Southern blotting, liquid (2x concentrate)

description

for Northern and Southern blotting, liquid (2x concentrate)

description

pH 7.0±0.05 (25 °C), BioXtra, Molecular Biology, 3 M, non-sterile; 0.2 μm filtered

description

pH 5.2±0.1 (25 °C), Molecular Biology, 3 M, 0.2 μm filtered

Quality Level

100, 200

Quality Level

100

Quality Level

200

Quality Level

200

form

liquid (2x concentrate)

form

liquid (2x concentrate)

form

liquid

form

liquid

storage temp.

-10 to -25°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

-

foreign activity

-


Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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