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Merck

T3411

Tetramethylammonium chloride solution

Molecular Biology

Synonym(s):

N,N,N-Trimethylmethanaminium chloride

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500 ML

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About This Item

CAS Number:
75-57-0
NACRES:
NA.31
PubChem Substance ID:
24900143
UNSPSC Code:
12352107
MDL number:
MFCD00011628

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Product Name

Tetramethylammonium chloride solution, Molecular Biology

InChI

1S/C4H12N.ClH/c1-5(2,3)4;/h1-4H3;1H/q+1;/p-1

SMILES string

[Cl-].C[N+](C)(C)C

InChI key

OKIZCWYLBDKLSU-UHFFFAOYSA-M

grade

Molecular Biology

concentration

5 M

foreign activity

DNase, RNase, none detected

Quality Level

200

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This Item
8661486870334901
Tetramethylammonium chloride solution Molecular Biology

Sigma-Aldrich

T3411

Tetramethylammonium chloride solution

Tetraethylammonium chloride BioUltra, Molecular Biology, ≥99.0% (AT)

Sigma-Aldrich

86614

Tetraethylammonium chloride

Tetrabutylammonium chloride ≥97.0% (NT)

Sigma-Aldrich

86870

Tetrabutylammonium chloride

Tetramethylammonium hydroxide solution 25 wt. % in methanol

Sigma-Aldrich

334901

Tetramethylammonium hydroxide solution

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

concentration

5 M

concentration

-

concentration

-

concentration

25 wt. % in methanol

foreign activity

DNase, RNase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

Application

Tetramethylammonium chloride solution (TMAC) has been used:
  • in the preparation of hybridization cocktail for array hybridization and scanning
  • in next-generation sequencing (NGS), and genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) library preparation
  • in the preparation of TMAC buffer and bead hybridization mixture for hybridization and detection

General description

Tetramethylammonium binds AT-rich DNA polymers while concomitantly abolishing the preferential melting of AT versus GC base pairs. It is supplied as a 0.2 μm filtered solution in 18 megohm water.

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Certificates of Analysis (COA)

Lot/Batch Number
0000447610
0000447611
0000447611
0000447610
0000425593

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Yanwen Guo et al.
Methods in molecular biology (Clifton, N.J.), 1176, 33-44 (2014-07-18)
As small noncoding RNAs, microRNAs (miRNAs) regulate diverse biological functions, including physiological and pathological processes. The expression and deregulation of miRNA levels contain rich information with diagnostic and prognostic relevance and can reflect pharmacological responses. The increasing interest in miRNA-related
Nikolay L Malinin et al.
Nature protocols, 16(12), 5592-5615 (2021-11-14)
Genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) is a sensitive, unbiased, genome-wide method for defining the activity of genome-editing nucleases in living cells. GUIDE-seq is based on the principle of efficient integration of an end-protected double-stranded oligodeoxynucleotide
W B Melchior et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(2), 298-302 (1973-02-01)
Several small alkylammonium ions can eliminate, or even reverse, the usual dependence of the DNA transition temperature on base composition. For example, in 3 M tetramethylammonium chloride, or 2.4 M tetraethylammonium chloride, DNAs of different base compositions all melt at
Hybridization of genomic DNA to oligonucleotide probes in the presence of tetramethylammonium chloride.
A G DiLella et al.
Methods in enzymology, 152, 447-451 (1987-01-01)
Xiao-Yong Li et al.
Methods in molecular biology (Clifton, N.J.), 809, 3-26 (2011-11-25)
Immunoprecipitation of cross-linked chromatin in combination with microarrays (ChIP-chip) or ultra high-throughput sequencing (ChIP-seq) is widely used to map genome-wide in vivo transcription factor binding. Both methods employ initial steps of in vivo cross-linking, chromatin isolation, DNA fragmentation, and immunoprecipitation.
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Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. At what temperature is this product stored？

    1 answer

    1. This product may be stored in room temperature.

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