T8657

Trichloroacetic acid

suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%

• Synonym(s): TCA
• Linear Formula: Cl₃CCOOH
• CAS Number: 76-03-9
• Molecular Weight: 163.39
• UNSPSC Code: 41105319
• NACRES: NB.22
• PubChem Substance ID: 24900550
• EC Number: 200-927-2
• Beilstein/REAXYS Number: 970119
• MDL number: MFCD00004177

Properties

• Product Name: Trichloroacetic acid, suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• SMILES string: OC(=O)C(Cl)(Cl)Cl
• InChI key: YNJBWRMUSHSURL-UHFFFAOYSA-N
• grade: electrophoresis grade
• vapor density: <1 (vs air)
• vapor pressure: 1 mmHg ( 51 °C)
• assay: ≥99%
• form: powder or flakes (or crystals)
• technique(s): electrophoresis: suitable
• turbidity: ≤3 NTU (1 g/ml)
• refractive index: n20/D 1.62 (lit.)
• bp: 196 °C (lit.)
• mp: 54-58 °C (lit.)
• solubility: water: 1 g/mL, clear, colorless
• density: 1.62 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤0.001%; nitrate (NO₃^-): ≤0.002%; phosphate (PO₄^3-): ≤5 ppm; sulfate (SO₄^2-): ≤0.02%
• cation traces: Fe: ≤0.0005%; heavy metals (as Pb): ≤0.002%
• suitability: suitable for fixing solution (for IEF and PAGE gels)
• application(s): cell analysis
• storage temp.: 2-8°C
• Quality Level: 200

Description

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Trichloroacetic acid has been used:

• for hydrolysis of DNA isolated from the cell[1]
• for precipitation of glutathione isolated from seminal plasma[2]
• as a fixative in microscopy[3]

General description

Trichloroacetic acid (TCA) is produced during the chlorination of water comprising natural organic material.[4] It is a novel reagent used in clinical laboratories for deproteinization of body fluids.[5] In addition, TCA is also used in the treatment of genital warts[6] and acne scars.[7]

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H314,H410
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• wgk: WGK 2
• flash_point_f: >235.4 °F - closed cup
• flash_point_c: > 113 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges