The UPS FASTA file is accessible from the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/protein-mass-spectrometry/ups1-and-ups2-proteomic
UPS2
Proteomics Dynamic Range Standard Set
Protein Mass Spectrometry Calibration Standard
Synonym(s):
Dynamic Range Standards, Proteomics Standard Set
Sign In to View Organizational & Contract Pricing
Select a Size
1 SET
$1,310.00
5 X 1 SET
$5,280.00
About This Item
Skip To
biological source
human
form
ready-to-use solution
quality
Protein Mass Spectrometry Calibration Standard
concentration
10.6 μg/ampule protein
technique(s)
mass spectrometry (MS): suitable
shipped in
wet ice
storage temp.
−20°C
Related Categories
1 of 4
This Item | UPS1 | EMS0004 | EMS0005 |
|---|---|---|---|
| technique(s) mass spectrometry (MS): suitable | technique(s) mass spectrometry (MS): suitable | technique(s) - | technique(s) - |
| biological source human | biological source - | biological source - | biological source - |
| concentration 10.6 μg/ampule protein | concentration - | concentration - | concentration - |
| form ready-to-use solution | form ready-to-use solution | form ready-to-use solution | form ready-to-use solution |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.
Application
Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins,[1] embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes.[2] It has also been used as a standard to spike HeLa cells for label-free quantification.[3]
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]
Kit Components Also Available Separately
Product No.
Description
SDS
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Boumediene Soufi et al.
Frontiers in microbiology, 6, 103-103 (2015-03-06)
We set out to provide a resource to the microbiology community especially with respect to systems biology based endeavors. To this end, we generated a comprehensive dataset monitoring the changes in protein expression, copy number, and post translational modifications in
Björn Schwanhäusser et al.
Nature, 473(7347), 337-342 (2011-05-20)
Gene expression is a multistep process that involves the transcription, translation and turnover of messenger RNAs and proteins. Although it is one of the most fundamental processes of life, the entire cascade has never been quantified on a genome-wide scale.
Taejoon Kwon et al.
Journal of proteome research, 10(7), 2949-2958 (2011-04-15)
Shotgun proteomics using mass spectrometry is a powerful method for protein identification but suffers limited sensitivity in complex samples. Integrating peptide identifications from multiple database search engines is a promising strategy to increase the number of peptide identifications and reduce
Jürgen Cox et al.
Molecular & cellular proteomics : MCP, 13(9), 2513-2526 (2014-06-20)
Protein quantification without isotopic labels has been a long-standing interest in the proteomics field. However, accurate and robust proteome-wide quantification with label-free approaches remains a challenge. We developed a new intensity determination and normalization procedure called MaxLFQ that is fully
Rosemary Yu et al.
Nature communications, 11(1), 1881-1881 (2020-04-22)
Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwise reducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and
Articles
High-throughput proteomics advances with improved analysis methods and mass spectrometry.
Related Content
Instructions
Standardize research with Universal and Dynamic Proteomics Standards, complex and well-characterized reference standards for mass spectrometry.
-
Regarding SKU# UPS2, can you please send me FASTA file of 48 proteins in UPS2 product? thanks you
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service