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PC548
Anti-Porin (Ab-5) (185-197) Rabbit pAb
liquid, Calbiochem®
Sinónimos:
Anti-VDAC (Voltage-Dependent Anion Channel)
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NACRES:
NA.43
UNSPSC Code:
12352203
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, rat
Application:
—
Citations:
19
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Servicio técnico
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Permítanos ayudarlebiological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (2 µg/mL))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VDAC1(7416)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~31 kDa (VDAC) porin protein.
Recognizes the ~31 kDa porin protein.
This Anti-Porin (Ab-5) (185-197) Rabbit pAb is validated for use in Immunoblotting for the detection of Porin (Ab-5) (185-197).
Immunogen
Human
a synthetic peptide corresponding to amino acids 185-197 of human porin
Application
Immunoblotting (2 g/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1 mg/ml BSA.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Rat heart tissue
Rat heart tissue
Other Notes
Crompton, M., et al. 1999. Biochem. J.341, 233.
Yu, W.H., et al. 1995. J. Biol. Chem.270, 13998.
McEnry, M.W., et al. 1993. J. Biol. Chem.268, 23289.
Yu, W.H., et al. 1995. J. Biol. Chem.270, 13998.
McEnry, M.W., et al. 1993. J. Biol. Chem.268, 23289.
The immunogen peptide is 100% conserved in all three isoforms of VDAC (VDAC1, VDAC2, and VDAC3). Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
1 of 1
Este artículo | ||
|---|---|---|
| description liquid, Calbiochem® | description clone N152B/23, from mouse | description recombinant, expressed in HEK 293 cells |
| antibody form affinity isolated antibody | antibody form purified antibody | antibody form purified antibody |
| biological source rabbit | biological source mouse | biological source rabbit (recombinant) |
| Quality Level 100 | Quality Level 100 | Quality Level 200 |
| storage temp. −20°C | storage temp. - | storage temp. 2-8°C |
| species reactivity human, rat | species reactivity rat, mouse, human | species reactivity mouse, human, rat |
| Gene Information human ... VDAC1(7416) | Gene Information human ... VDAC1(7416) | Gene Information mouse ... Vdac1(22333) |
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I have used this antibody but I not only get a band at ~35kDa. I get a stronger band at ~100kDa. I just wanted to know if this is something that is known to happen with this antibody and what this off-target band might be. Many thanks for any help.
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