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10108090001

Roche

Peroxidase (POD)

Name: Peroxidase (POD)
Brand: ROCHE

grade I, from horseradish

Sinónimos:

POD, peroxidase

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UNSPSC Code:

12352204

Número CE:

1.11.1.7 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg

Biological source:

horseradish

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Propiedades

biological source

horseradish

form

lyophilized (salt-free)

specific activity

≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg

packaging

pkg of 25,000 U

manufacturer/tradename

Roche

technique(s)

protein detection: suitable

optimum pH

6.0-6.5

shipped in

wet ice

storage temp.

2-8°C

Descripción

General description

Donor:hydrogen-peroxide oxidoreductase

Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.

Application

Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.

Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H₂O₂ produced from disaggregated slug cells.

Biochem/physiol Actions

Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.

Preparation Note

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).

Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.

Analysis Note

Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase
Purity number: approximately 3.0 (A₄₀₃/A₂₇₅)

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Este artículo	10814407001	11428861001	11582950001
Roche 10108090001 Peroxidase (POD)	Roche 10814407001 Peroxidase (POD)	Roche 11428861001 Peroxidase (POD), activated	Roche 11582950001 BM Chemiluminescence ELISA Substrate (POD)
description grade I, from horseradish	description EIA grade, from horseradish	description from horseradish	description -
technique(s) protein detection: suitable	technique(s) protein detection: suitable, activity assay: suitable	technique(s) protein detection: suitable	technique(s) protein detection: suitable, ELISA: suitable
biological source horseradish	biological source horseradish	biological source horseradish	biological source -
specific activity 250 U/mg, ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.)	specific activity ~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2)	specific activity 800 units/mg protein (at 25 °C with ABTS and H₂O₂ as substrate (pH 5.0).)	specific activity -
form lyophilized (salt-free)	form lyophilized (salt-free)	form lyophilized	form solution
storage temp. 2-8°C	storage temp. −20°C (−15°C to −25°C)	storage temp. 2-8°C	storage temp. 2-8°C
optimum pH 6.0-6.5	optimum pH 6.0-6.5	optimum pH 6.0-6.5	optimum pH -