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Merck

CS0760

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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1 KIT

US$ 827,00

US$ 827,00

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EC Number:
217-896-6
UNSPSC Code:
12352207
NACRES:
NA.32

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Quality Level

200

usage

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

flow cytometry: suitable
protein staining: suitable

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

dry ice

storage temp.

−20°C

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Este artículo
CS0720CS0390RPROB
Isolated Mitochondria Staining Kit 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

Sigma-Aldrich

CS0760

Isolated Mitochondria Staining Kit

Citrate Synthase Assay Kit 1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

Sigma-Aldrich

CS0720

Citrate Synthase Assay Kit

Mitochondria Staining Kit 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

Sigma-Aldrich

CS0390

Mitochondria Staining Kit

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

Sigma-Aldrich

RPROB

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

technique(s)

flow cytometry: suitable, protein staining: suitable

technique(s)

-

technique(s)

flow cytometry: suitable, protein staining: suitable

technique(s)

protein staining: suitable

detection method

fluorometric

detection method

-

detection method

fluorometric

detection method

-

usage

 kit sufficient for 1000 reactions (using 96 multiwell plates),  kit sufficient for 50 reactions (in a 2 mL cuvette)

usage

 kit sufficient for 100 reactions (using a 1 ml cuvette),  kit sufficient for 480 reactions (using 96 multiwell plates)

usage

 kit sufficient for 200 tests (of 1 mL cell suspensions),  kit sufficient for 40 tests (of 5 mL cell suspensions)

usage

sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

fluorescence

-

fluorescence

λex 490 nm; λem 530 nm (green) (JC-1 monomers), λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

fluorescence

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

-

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.[1][2][3]

Biochem/physiol Actions

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Features and Benefits

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Analysis Note

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Solo componentes del kit

Referencia del producto
Descripción
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000275141
0000275141
11180552
108M4080V
038M4176V

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Neuroprotective efficacy of mitochondrial antioxidant MitoQ in suppressing peroxynitrite-mediated mitochondrial dysfunction inflicted by lead toxicity in the rat brain
Maiti AK, et al.
Neurotoxicity Research, 31(3), 358-372 (2017)
Arpan Kumar Maiti et al.
Interdisciplinary toxicology, 11(4), 306-315 (2019-11-26)
Nickel is a potential neurotoxic pollutant inflicting damage in living organisms, including fish, mainly through oxidative stress. Previous studies have demonstrated the impact of nickel toxicity on mitochondrial function, but there remain lacunae on the damage inflicted at mitochondrial respiratory
Subcellular fractionation of mitochondria.
Rice, J.E., and Lindsay, J.G. et al.
Subcellular Fractionation, A Practical Approach, 107- 142 (1997)
Joanna Skalska et al.
Neurotoxicity research, 38(3), 650-664 (2020-06-27)
Extensive incorporation of silver nanoparticles (AgNPs) into many medical and consumer products has raised concerns about biosafety. Since nanosilver accumulates persistently in the central nervous system, it is important to assess its neurotoxic impacts. We investigated a model of prolonged
Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse
Wu G, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1801(4), 446-454 (2010)
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