F4274

Fluorescein isothiocyanate isomer I

≥97.5% purity (HPLC), powder

• Sinónimos: FITC, Fluorescein 5-isothiocyanate
• Fórmula empírica (notación de Hill): C₂₁H₁₁NO₅S
• Número CAS: 3326-32-7
• Peso molecular: 389.38
• Beilstein/REAXYS Number: 1407295
• EC Number: 222-042-0
• MDL number: MFCD00005063
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894860
• NACRES: NA.47

Propiedades

• Nombre del producto: Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
• Quality Level: 100
• assay: ≥97.5% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to very dark yellow
• mp: >360 °C (lit.)
• solubility: acetone: soluble 10 mg/mL
• fluorescence: λ_ex 492 nm; λ_em 518 nm (green)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Descripción

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]

Application

El reactivo para el marcado con FITC de proteínas; microsecuenciado de proteínas y péptidos (HPLC)

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.[3][4]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Información de seguridad

• Pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves