Saltar al contenido
Merck

Utilization of by-products derived from bioethanol production process for cost-effective production of lactic acid.

Journal of industrial microbiology & biotechnology (2014-08-29)
Se-Kwon Moon, Young-Jung Wee, Gi-Wook Choi
RESUMEN

The by-products of bioethanol production such as thin stillage (TS) and condensed distillers solubles (CDS) were used as a potential nitrogen source for economical production of lactic acid. The effect of those by-products and their concentrations on lactic acid fermentation were investigated using Lactobacillus paracasei CHB2121. Approximately, 6.7 g/L of yeast extract at a carbon source to nitrogen source ratio of 15 was required to produce 90 g/L of lactic acid in the medium containing 100 g/L of glucose. Batch fermentation of TS medium resulted in 90 g/L of lactic acid after 48 h, and the medium containing 10 % CDS resulted in 95 g/L of lactic acid after 44 h. Therefore, TS and CDS could be considered as potential alternative fermentation medium for the economical production of lactic acid. Furthermore, lactic acid fermentation was performed using only cassava and CDS for commercial production of lactic acid. The volumetric productivity of lactic acid [2.94 g/(L·h)] was 37 % higher than the productivity obtained from the medium with glucose and CDS.

MATERIALES
Número de producto
Marca
Descripción del producto

Sigma-Aldrich
Alcohol etílico puro 200, Molecular Biology
Sigma-Aldrich
Alcohol etílico puro, 200 proof, ACS reagent, ≥99.5%
Sigma-Aldrich
Alcohol etílico puro, 200 proof, meets USP testing specifications
Sigma-Aldrich
D-(+)-Glucosa, ≥99.5% (GC)
Sigma-Aldrich
D-(+)-Glucosa, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
Alcohol etílico puro, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
D-(+)-Glucosa solution, 45% in H2O, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
Alcohol etílico puro 190, for molecular biology
Sigma-Aldrich
Dextrosa, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications
Sigma-Aldrich
D-(+)-Glucosa solution, 100 g/L in H2O, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
Alcohol etílico puro, 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
D-(+)-Glucosa, ≥99.5% (GC), BioXtra
Supelco
D-(+)-Glucosa, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Lactic acid, meets USP testing specifications
USP
D-(+)-Glucosa, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Lactic acid, 88%, FCC
Sigma-Aldrich
D-(+)-Glucosa, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
Supelco
Etanol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
D-(+)-Glucosa, ACS reagent
Supelco
D-(+)-Glucosa, analytical standard
Sigma-Aldrich
Lactic acid, natural, ≥85%
Sigma-Aldrich
Lactic acid solution, ACS reagent, ≥85%
Supelco
Etanol, standard for GC
Sigma-Aldrich
L-(−)-Glucose, ≥99%
Sigma-Aldrich
DL-Lactic acid, ~90% (T)
Supelco
Lactic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Reagent Alcohol, anhydrous, ≤0.003% water
Sigma-Aldrich
D-(+)-Glucosa, suitable for mouse embryo cell culture, ≥99.5% (GC)
Sigma-Aldrich
Reagent Alcohol, anhydrous, ≤0.005% water
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol