07-2126

Anti-phospho-FOXO1 Antibody (Thr24)

from rabbit, purified by affinity chromatography

• Synonym(e): Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_002006
• UniProt-Hinterlegungsnummer: Q12778
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pThr24)
• Angaben zum Gen: human ... FOXO1(2308)

Beschreibung

Allgemeine Beschreibung

Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at Thr24.

~78 kDa observed.
Calculated molecular weight is 70 kDa FOXO1 has been shown to migrate to ~78 kDa in western blots (Kamei, Y., et al. (2004). JBC. 279(39):41114-41123).

Immunogen

Epitope: Phosphorylated Thr24

KLH-conjugated linear peptide corresponding to human FOXO1 phosphorylated at Thr24.

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Transkriptionsfaktoren

Use Anti-phospho-FOXO1 (Thr24) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-FOXO1 (Thr24) also known as Forkhead box protein O1.

Biochem./physiol. Wirkung

This antibody recognizes FOXO1 phosphorylated at Thr24.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Angaben zur Herstellung

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
HEK293 cell lysate untreated and BMP2-treated

Evaluated by Western Blot in HEK293 cell lysate untreated and BMP2-treated.

Western Blot Analysis: 2 µg/mL of this antibody detected phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable