ABC2044

Anti-Muc2-D3

• Synonym(e): Mucin-2 D3 domain
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: EM, WB
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: calculated mol wt 550.85 kDa, observed mol wt ~60 kDa
• purified by: using protein G
• species reactivity: human
• species reactivity (predicted by homology): mouse
• packaging: antibody small pack of 100
• technique(s): electron microscopy: suitable, western blot: suitable
• isotype: IgGκ
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_002448.4
• UniProt accession no.: Q02817
• storage temp.: -10 to -25°C
• target post-translational modification: unmodified
• Gene Information: human ... MUC2(4583)

Beschreibung

General description

Mucin-2 (UniProt: Q02817; also known as MUC-2, Intestinal Mucin-2) is encoded by the MUC2 (also known as SMUC) gene (Gene ID: 4583) in human. Mucin-2 (MUC2) is a secreted protein that coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. It provides a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Mucin-2 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature form. It exists as a disulfide-linked homotrimer that can be highly O-glycosylated. It contains four VWFD domains and two VWFC domains. Mucin-2 undergoes proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer that allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. The numerous O-glycans on the Mucin-2 are reported to serve as nutrients for the bacteria, serve as attachment sites, and probably contribute to the selection of the species-specific colon flora. Reduced levels of Mucin-2 have been linked to the early stage of colorectal cancer development, which is caused by methylation of the MUC2 promoter. This polyclonal antibody detects D3 domain of mucin-2, which is localized in amino acids 858-1259. (Ref.: Wi, D-H., et al. (2021). BMB Rep. 54(7); 344-355; Javitt, G., et al. (2020). Cell. 183(3); 717-729; Javitt, G., et al. (2019). J. Mol. Biol. 431(19); 3740-3752; Johansson, MEV., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(Suppl. 1); 4659-4665).

Immunogen

His-tagged recombinant fragment corresponding to 402 amino acids from the D3 domain of human Mucin-2.

Application

Quality Control Testing

Evaluated by Western Blotting with Human Muc2-D3 protein.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Human Muc2-D3 protein.

Tested Applications

Electron Microscopy: A representative lot detected Human Mucin-2 protein in Electron Microscopy applications (Javitt, G., et. al. (2020). Cell.183(3): 717-729).

Western Blotting Analysis: A representative lot detected Human Mucin-2 protein in Western Blotting applications (Javitt, G., et. al. (2020). Cell. 183(3): 717-729).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

This rabbit polyclonal antibody detects D3 domain of Mucin-2. It targets an epitope within D3 domain from the N-terminal half.

Physical form

Purified rabbit polyclonal antibody in PBS without azide.

Preparation Note

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable