MABC182

Anti-NRF-1 Antibody, clone 10B11.1

clone 10B11.1, from mouse

• Synonym(e): Nuclear respiratory factor 1, Alpha-pal, Alpha palindromic-binding protein, NRF-1
• UNSPSC Code: 12352203
• NACRES: NA.43
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 10B11.1, monoclonal
• Application: WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 10B11.1, monoclonal
• species reactivity: mouse
• species reactivity (predicted by homology): human (based on 100% sequence homology)
• technique(s): western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001035199
• UniProt accession no.: Q16656
• target post-translational modification: unmodified
• Gene Information: human ... NRF1(4899)

Beschreibung

General description

Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (Also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

~58 kDa observed. 53.54/53.57 kDa (human/mouse isoform Long), 46.16 kDa (human/mouse isoform Short), 36.05 kDa (human isoform 3), 55.67 kDa (human isoform 4) calculated.

Immunogen

Epitope: Internal (N-terminal half).

GST-tagged recombinant human NRF-1 internal fragment.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Transcription Factors

This Anti-NRF-1 Antibody, clone 10B11.1 is validated for use in Western Blotting for the detection of NRF-1.

Biochem/physiol Actions

Immunogen construct region is present in human NRF-1 spliced isoform Long, isfoform Short, and isfoform 4, but is more than 40% absent from spliced isoform 4 reported by UniProt (Q16656).

Physical form

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected NRF-1 in 10 µg of NIH/3T3 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1