MABE428

Anti-WDR5 Antibody, clone 7B11

ascites fluid, clone 7B11, from mouse

• Synonym(e): WD repeat-containing protein 5, BMP2-induced 3-kb gene protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 7B11, monoclonal
• Species reactivity: human
• Application: WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 7B11, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG2b
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WDR5(11091)

Beschreibung

General description

WD repeat-containing protein 5 (WDR5) or alternatively BMP2-induced 3kb gene protein, is encoded by the human gene named WDR5/BIG3 and is part of a family of WD-40 repeat proteins that appear to facilitate the formation of multi-protein complexes much like a sticky tape will bind a variety of components together. The WD repeat proteins are involved in a wide array of cellular functions and processes including cell cycle progression, homeobox gene activation, apoptosis, gene regulation and histone methylation. One of WDR5 best studied activities is as part of a multi-protein complex that facilitates the methylation of histones, particularly histone H3, through its interaction with MLL a histone methyltransferase. WDR5 is also expressed in developing bone cells including osteoblasts, chondrocytes, and marrow stromal cells and works to accelerate osteoblast differentiation and positively promotes Wnt signaling in developing bone tissues. EMD-Millipore’s Anti-WDR5 mouse monoclonal antibody has been tested in western blot using HeLa cell lysates.

~40 kDa observed

Immunogen

Purified recombinant fragment of human WDR5 expressed in E. Coli.

Application

Anti-WDR5 Antibody, clone 7B11 is a highly specific mouse monoclonal antibody & that targets WDR5 & has been tested in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Physical form

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cell lysate

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected WDR5 in HeLa cell lysate.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable