OP92

Anti-BRCA1 (Ab-1) Mouse mAb (MS110)

liquid, clone MS110, Calbiochem^®

• Synonym(e): Anti-Breast Cancer Susceptibility Protein
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: MS110, monoclonal
• Species reactivity: human
• Application: —
• Citations: 118

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MS110, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Frozen Sections ; Immunoblotting (2 µg/mL); Immunofluorescence ; Immunoprecipitation ; Paraffin Sections )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... BRCA1(672)

Beschreibung

General description

Anti-BRCA1 (Ab-1), mouse monoclonal, clone MS110, recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. It is validated for WB, IF, IP, and frozen and paraffin sections.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.

Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not cross-react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

Immunogen

Epitope: within the N-terminal 304 amino acids of BRCA1

Human

recombinant human BRCA1

Application

Frozen Sections (see application reference)

Immunoblotting (2 µg/ml)

Immunofluorescence (see application reference)

Immunoprecipitation (see application reference)

Paraffin Sections (see application reference)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
MCF7 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.

Scully, R., et al. 1996. Science272, 123.
Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.
Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.
Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113.
Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539.
Merajver, S.D., et al. 1995. Nat. Genet.9, 439.
Struewing, J.P., et al. 1995. Nat. Genet.11, 198.
Thompson, M.E., et al. 1995. Nat. Genet.9, 444.
Futreal, P.A., et al. 1994. Science266, 120.
Miki, Y., et al. 1994. Science266, 66.
Wilson, C.A., et al. 1999. Nat. Genet.21, 236.

Legal Information

Sold under license of U.S. Patents 5,753,441 and 6,162,897.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable